| Yazarlar |
Aysel Kalaycı Yiğin
İstanbul Üniversitesi-Cerrahpaşa, Türkiye |
|
Hüseyin Dönmez
|
Doç. Dr. Mustafa HİTİT
Türkiye |
|
Sena Seven
|
|
Nadire Eser
Türkiye |
|
Ercan Kurar
Türkiye |
|
Mehmet Seven
Türkiye |
| Özet |
| Background/Aim: We aimed to investigate the in vitro modulating effects of medicarpin on the PI3K/AKT signal pathway gene expressions in head and neck squamous cell carcinoma (HNSCC). Materials and Methods: The effect of medicarpin on PTEN and other associated genes in the PTEN/AKT signal pathway was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, real-Time quantitative polymerase chain reaction, and Western blot analysis in the SCCL-MT1 (HNSCC) and control (HEK-293) cell lines. Results: The IC50 dose was 80 μM as a result of medicarpin treatment on HNSCC cells (P = 0.0006). It was found that PTEN and AKT gene expressions increased after the medicarpin administration while PDK1 gene expression was decreased in SCCL-MT1 cells (P = 0.0002, P = 0.0003, and P = 0.05, respectively). Protein expression results showed that medicarpin-Treated cells significantly increased in pAKT (P = 0.024), pPTEN (P = 0.032), and decreased pPDK1 (P = 0.059) in SCCL-MT1. Conclusions: Our data show that medicarpin modulates HNSCC cells by increasing the PTEN and decreasing PDK1 expressions. PDK1 gene expression effects mTOR pathway which may increase AKT gene. Our study suggests that both medicarpin extracts combination with the HNSCC drug may be more effective in cancer treatment. Future prospective studies that integrate molecular and pharmacogenetic studies are crucial for revealing the mechanism and preventive medical efforts. |
| Anahtar Kelimeler |
| Head and neck squamous cell carcinoma | in vitro | medicarpin | PTEN/AKT signal pathway |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF CANCER RESEARCH AND THERAPEUTICS |
| Dergi ISSN | 0973-1482 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2022 |
| Cilt No | 18 |
| Sayı | 1 |
| Sayfalar | 180 / 184 |
| Doi Numarası | 10.4103/jcrt.jcrt_641_21 |
| Makale Linki | http://dx.doi.org/10.4103/jcrt.jcrt_641_21 |
| Atıf Sayıları | |
| WoS | 2 |
| SCOPUS | 2 |
