| Yazarlar |
Holy C. Evans
|
|
Thu Tran Nhat Dınh
|
|
Muhammet Raşit Uğur
|
Doç. Dr. Mustafa HİTİT
Türkiye |
|
Dıshnu Sajeev
|
|
Abdullah Kaya
Selçuk Üniversitesi, Türkiye |
|
Eınko Topper
|
|
Molly C. Nıcodemus
|
|
Gary D. Smıth
|
|
Erdogan Memılı
|
| Özet |
| The objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor; n = 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform–methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography–mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P < 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg; P < 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Scientific Reports |
| Dergi ISSN | 2045-2322 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2020 |
| Cilt No | 10 |
| Doi Numarası | 10.1038/s41598-020-77089-9 |
| Makale Linki | http://dx.doi.org/10.1038/s41598-020-77089-9 |
