Lipidomic markers of sperm cryotolerance in cattle
Yazarlar (10)
Holy C. Evans
Thu Tran Nhat Dınh
Muhammet Raşit Uğur
Mustafa Hitit
Kastamonu Üniversitesi, Türkiye
Kastamonu Üniversitesi, Türkiye
Dıshnu Sajeev
Prof. Dr. Abdullah Kaya Selçuk Üniversitesi, Türkiye
Eınko Topper
Molly C. Nıcodemus
Gary D. Smıth
Erdogan Memılı
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | SCIENTIFIC REPORTS (Q1) | ||
| Dergi ISSN | 2045-2322 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 11-2020 |
| Kabul Tarihi | 01-11-2020 | Yayınlanma Tarihi | 19-11-2020 |
| Cilt / Sayı / Sayfa | 10 / 1 / – | DOI | 10.1038/s41598-020-77089-9 |
| Makale Linki | https://www.nature.com/articles/s41598-020-77089-9.pdf | ||
| UAK Araştırma Alanları |
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| Özet |
| The objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor; n = 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform–methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography–mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P < 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg; P < 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |