Alcoholic extract of Tarantula cubensis induces apoptosis in MCF-7 cell line
Yazarlar (6)
Ayşe Er
Selçuk Üniversitesi, Türkiye
Orhan Çorum
Hatay Mustafa Kemal Üniversitesi, Türkiye
Duygu Durna Çorum
Hatay Mustafa Kemal Üniversitesi, Türkiye
Aydın Güzeloğlu
Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | BIOMEDICAL RESEARCH-INDIA | ||
| Dergi ISSN | 0970-938X Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2017 |
| Cilt / Sayı / Sayfa | 28 / 8 / 3660–3665 | DOI | – |
| Makale Linki | https://www.biomedres.info/archive/aabmr-volume-28-issue-8-year-2017.html | ||
| Özet |
| Tarantula cubensis Alcoholic Extract (TCAE) is a homeopathic agent used for treating many disorders. This study aimed to define the effects of TCAE on the breast carcinoma cell line (MCF-7). After various concentrations (10, 20, 40, 80 and 160 µl/ml) of TCAE were applied to MCF-7 cells and the human embryonic kidney cell line (HEK293), the cells were incubated for 1, 3, 6, 9, 12, 24 and 48 h, followed by analysis by MTT assays. According to the results of the MTT assays, cells treated with 20 or 40 µl/ml TCAE for 6 h were applied to apoptosis analysis by flow cytometry. Secreted levels of tumor necrosis factor alpha (TNFα), interleukin (IL)-1β, IL-6, IL-10, Interferon-γ (IFNγ), Transforming Growth Factor-β (TGFβ), and Nuclear Factor-kappa B (NF-κB) were measured using ELISAs. TNFα and TGFβ levels increased while IL-6 and IL-10 levels fluctuated in MCF-7 cells. In conclusion, our study suggests that TCAE may change the normal cancer physiology and lead to cell death by activating apoptosis in MCF-7 cells. |
| Anahtar Kelimeler |
| Apoptosis | Cytokines | MCF-7 | Tarantula cubensis alcoholic extract | Theranekron |
