Investigation of interferon-tau stimulated genes (ISGs) simultaneously in the endometrium, corpus luteum (CL) and peripheral blood leukocytes (PBLs) in the preluteolytic stage of early pregnancy in ewes
Yazarlar (11)
Zekeriya Kıyma Eskişehir Osmangazi Üniversitesi, Türkiye
Prof. Dr. Mehmet Köse Dicle Üniversitesi, Türkiye
Prof. Dr. Mehmet Osman Atlı Harran Üniversitesi, Türkiye
Çağlayan Özel
Türkiye
Türkiye
Mustafa Hitit
Gonca Sönmez Selçuk Üniversitesi, Türkiye
Muhammet Kaya Eskişehir Osmangazi Üniversitesi, Türkiye
Mehmet Salih Kaya Ankara Yıldırım Beyazıt Üniversitesi, Türkiye
Prof. Dr. Ercan Kurar Necmettin Erbakan Üniversitesi, Türkiye
Seyit Ali Kayış Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Aydın Güzeloğlu
Türkiye
Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Small Ruminant Research (Q2) | ||
| Dergi ISSN | 0921-4488 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2016 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-07-2016 |
| Cilt / Sayı / Sayfa | 140 / 1 / 1–27 | DOI | 10.1016/j.smallrumres.2016.05.007 |
| Makale Linki | http://dx.doi.org/10.1016/j.smallrumres.2016.05.007 | ||
| UAK Araştırma Alanları |
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| Özet |
| The aim of the present study was to investigate the expression profiles of Interferon-tau (IFN-τ) stimulated genes (ISGs) at the mid-luteal stage of the cycle (on day 13) in the uterine endometrium and extra-uterine tissues, such as the corpus luteum (CL), and in the peripheral blood leukocytes (PBLs) of pregnant and non-pregnant ewes. The objective was to evaluate the possibility of using the regulation of ISG expression in PBLs as a possible early pregnancy indicator in ruminants. For this purpose, multiparous ewes were synchronized and either allowed to mate (pregnancy group) or detected in estrus (cyclic group; day 0). The ewes were slaughtered on day 13, and the PBLs, endometrium and luteal tissues were collected. Total RNA was isolated from eight cyclic and eight pregnant ewes, and qPCR was employed to detect the steady state levels of Interferon-stimulated gene 15 (ISG15), Myxovirus (influenza virus) resistance 1 (Mx1) and Receptor transporter protein 4 (RTP4) mRNAs. The expressions of ISG15, Mx1 and RTP4 were detected in the endometrium, CL and PBLs on day 13 of the estrous cycle and pregnancy. The expressions of these ISGs were upregulated only in the endometrium of pregnant ewes compared to non-pregnant ewes, but this stimulation was not observed in the CL and PBLs. The results suggest that the embryo stimulates ISGs only in the endometrium, and the effects are not evident in the extra-uterine tissues on day 13 of pregnancy. This study suggests that the measurement of the ISG expression in the PBLs is not a reliable detection method of early pregnancy in ewes, which are in the preluteolytic stage of early pregnancy. |
| Anahtar Kelimeler |
| Ewes | Interferon-stimulated genes | Pregnancy |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |