| Yazarlar |
Şengül Uysal
|
Dr. Öğr. Üyesi Aslı UĞURLU BAYARSLAN
Kastamonu Üniversitesi, Türkiye |
|
Gökhan Zengin
Selçuk Üniversitesi, Türkiye |
Prof. Dr. Mehmet Cengiz BALOĞLU
Kastamonu Üniversitesi, Türkiye |
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU
Kastamonu Üniversitesi, Türkiye |
|
Adriano Mollica
|
|
Luisa Custodio
|
|
Nuno R Neng
|
|
Jos Mf Nogueira
|
|
M Fawzi Mahomoodally
|
| Özet |
| Bidens tripartita L. is a traditional phyto-remedy used in several countries, yet there is still a paucity of data on its biological potential. We aimed to provide new insights on the pharmacological potential of extracts prepared from B. tripartita via highlighting its antioxidant, key enzymes inhibitory potency, and DNA protecting effects. Phytochemical profile was established using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) and bioactive compound(s) docked against target enzymes using in silico methods. Cytotoxicity against three cancer cell lines was assessed using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability test. The main compounds were luteolin-7-glucoside (cynaroside), chlorogenic acid, and epicatechin in the extracts. The methanol extract exhibited the highest radical scavenging activity. Ethyl acetate extract showed strongest α-amylase inhibitory activity, while the best α-glucosidase inhibitory effect recorded for the methanol extract. Molecular docking showed that cynaroside strongly interact to α-glucosidase cavity by establishing six hydrogen bonds. B. tripartita extracts were found to protect supercoiled form of pUC19 plasmid (>70%) and also showed anti-proliferative properties. Results amassed in the present study add on to a growing body of literature on the multi-pharmacological potency of B. tripartita which can be applied to bio-products development geared towards management of common diseases. |
| Anahtar Kelimeler |
| Anti-cancer | Anti-proliferative | Antioxidant | Cholinesterases | DNA protection | α-glucosidase |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | FOOD AND CHEMICAL TOXICOLOGY |
| Dergi ISSN | 0278-6915 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2018 |
| Cilt No | 111 |
| Sayı | 1 |
| Sayfalar | 525 / 536 |
| Doi Numarası | 10.1016/j.fct.2017.11.058 |
| Makale Linki | https://www.sciencedirect.com/science/article/pii/S027869151730738X?via3Dihub! |
| Atıf Sayıları | |
| WoS | 36 |
| SCOPUS | 40 |
| Google Scholar | 70 |
