Isolation of the 3β-HSD promoter from Digitalis ferruginea subsp. ferruginea and its functional characterization in Arabidopsis thaliana
 
Yazarlar (8)
Noreen Aslam
Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Muhammad Sameeullah Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Prof. Dr. Muhammet Yildirim Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Prof. Dr. Mehmet Cengiz BALOĞLU Kastamonu Üniversitesi, Türkiye
Buhara Yucesan
Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Andreas G. Lössl
Boku University, Avusturya
Mohammad Tahir Waheed
Quaid-İ-Azam University, Pakistan
Prof. Dr. Ekrem Gurel Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Molecular Biology Reports (Q4)
Dergi ISSN 0301-4851 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Makale Dili Türkçe Basım Tarihi 06-2022
Kabul Tarihi 24-05-2022 Yayınlanma Tarihi 22-06-2022
Cilt / Sayı / Sayfa 49 / 7 / 7173–7183 DOI 10.1007/s11033-022-07634-4
Makale Linki http://dx.doi.org/10.1007/s11033-022-07634-4
UAK Araştırma Alanları
Biyoteknoloji Moleküler Biyoloji
Özet
BackgroundAlthough members of the SDR gene family (short chain dehydrogenase) are distributed in kingdom of life, they have diverse roles in stress tolerance mechanism or secondary metabolite biosynthesis. Nevertheless, their precise roles in gene expression or regulation under stress are yet to be understood.MethodsAs a case study, we isolated, sequenced and functionally characterized the 3β-HSD promoter from Digitalis ferruginea subsp. ferruginea in Arabidopsis thaliana.ResultsThe promoter fragment contained light and stress response elements such as Box-4, G-Box, TCT-motif, LAMP element, ABRE, ARE, WUN-motif, MYB, MYC, W box, STRE and Box S. The functional analysis of the 3β-HSD promoter in transgenic Arabidopsis seedlings showed that the promoter was expressed in cotyledon and root elongation zone in 2 days’ seedlings. However, this expression was extended to hypocotyl and …
Anahtar Kelimeler
3β-HSD promoter | Abiotic stress | Functional characterization | SDR