| Yazarlar |
Musa Kavas
Ondokuz Mayıs Üniversitesi, Türkiye |
Prof. Dr. Mehmet Cengiz BALOĞLU
Kastamonu Üniversitesi, Türkiye |
|
Atabay Elif Seda
|
|
Tanman Ziplar Ummugulsum
|
|
Yıldız Daşgan Hayriye
|
|
Turgay Ünver
Çankırı Karatekin Üniversitesi, Türkiye |
| Özet |
| Members of basic helix-loop-helix (bHLH) gene family found in all eukaryotes play crucial roles in response to stress. Though, most eukaryotes carry the proteins of this family, biological functions of the most bHLH family members are not deeply evaluated in plants. In this study, we conducted a comprehensive genome-wide analysis of bHLH transcription factors in salt tolerant common bean. We identified 155 bHLH protein-encoding genes (PvbHLH) by using in silico comparative genomics tools. Based on the phylogenetic tree, PvbHLH genes were classified into 8 main groups with 21 subfamilies. Exon–intron analysis indicated that proteins belonging to same main groups exhibited a closely related gene structure. While, the PvbHLH gene family has been mainly expanded through segmental duplications, a total of 11 tandem duplication were detected. Genome-wide expression analysis of bHLH genes showed that 63 PvbHLH genes were differentially expressed in at least one tissue. Three of them displayed higher expression values in both leaf and root tissues. The in silico micro-RNA target transcript analyses revealed that totally 100 PvHLH genes targeted by 86 plant miRNAs. The most abundant transcripts, which were targeted by all 18 plant miRNA, were belonging to PvHLH-22 and PvHLH-44 genes. The expression of 16 PvbHLH genes in the root and leaf tissues of salt-stressed common bean was evaluated using qRT-PCR. Among them, two of PvbHLHs, PvbHLH-54, PvbHLH-148, were found to be up-regulated in both tissues in correlation with RNA-seq measurements. The results of this study could help improve understanding of biological functions of common bean bHLH family under salt stress. Additionally, it may provide basic resources for analyzing bHLH protein function for improving economic, agronomic and ecological benefit in common bean and other species. |
| Anahtar Kelimeler |
| bHLH gene family | Phaseolus vulgaris | Salt stress response | Transcription factor |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | MOLECULAR GENETICS AND GENOMICS |
| Dergi ISSN | 1617-4615 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2016 |
| Cilt No | 291 |
| Sayı | 1 |
| Sayfalar | 129 / 143 |
| Doi Numarası | 10.1007/s00438-015-1095-6 |
| Makale Linki | http://link.springer.com/10.1007/s00438-015-1095-6 |
| Atıf Sayıları | |
| WoS | 82 |
| SCOPUS | 89 |
| Google Scholar | 106 |
