Differential gene expression in liver tissues of streptozotocin induced diabetic rats in response to resveratrol treatment
Yazarlar (3)
Prof. Dr. Gökhan Sadi Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Prof. Dr. Mehmet Cengiz BALOĞLU Kastamonu Üniversitesi, Türkiye
Prof. Dr. Mehmet Bilgehan Pektaş Afyon Kocatepe Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | PLOS ONE | ||
| Dergi ISSN | 1932-6203 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 04-2015 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 23-04-2015 |
| Cilt / Sayı / Sayfa | 10 / 4 / 1–21 | DOI | 10.1371/journal.pone.0124968 |
| Makale Linki | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124968 | ||
| UAK Araştırma Alanları |
Biyoteknoloji
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| Özet |
| This study was conducted to elucidate the genome-wide gene expression profile in streptozotocin induced diabetic rat liver tissues in response to resveratrol treatment and to establish differentially expressed transcription regulation networks with microarray technology. In addition to measure the expression levels of several antioxidant and detoxification genes, real-time quantitative polymerase chain reaction (qRT-PCR) was also used to verify the microarray results. Moreover, gene and protein expressions as well as enzymatic activities of main antioxidant enzymes; superoxide dismutase (SOD-1 and SOD-2) and glutathione S-transferase (GST-Mu) were analyzed. Diabetes altered 273 genes significantly and 90 of which were categorized functionally which suggested that genes in cellular catalytic activities, oxidation-reduction reactions, co-enzyme binding and terpenoid biosynthesis were dominated by up-regulated expression in diabetes. Whereas; genes responsible from cellular carbohydrate metabolism, regulation of transcription, cell signal transduction, calcium independent cell-to-cell adhesion and lipid catabolism were down-regulated. Resveratrol increased the expression of 186 and decreased the expression of 494 genes in control groups. While cellular and extracellular components, positive regulation of biological processes, biological response to stress and biotic stimulants, and immune response genes were up-regulated, genes responsible from proteins present in nucleus and nucleolus were mainly down-regulated. The enzyme assays showed a significant decrease in diabetic SOD-1 and GST-Mu activities. The qRT-PCR and … |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 31 |
| Google Scholar | 52 |