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Expression Analysis of TaNAC69 1 and TtNAMB 2 Wheat NAC Family Transcription Factor Genes under Abiotic Stress Conditions in Durum Wheat Triticum turgidum       
Yazarlar
Prof. Dr. Mehmet Cengiz BALOĞLU Prof. Dr. Mehmet Cengiz BALOĞLU
Kastamonu Üniversitesi, Türkiye
Öz Mehmet Tufan
Hüseyin Avni Öktem
Konya Gıda ve Tarım Üniversitesi, Türkiye
Ayşe Meral Yücel
Orta Doğu Teknik Üniversitesi, Türkiye
Özet
NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. Compared to control samples, a quick response at the transcription level of TaNAC69-1 was observed after 3 h of salt treatment with a ninefold upregulation. Highest level of expression was observed at 24 and 48 h posttreatment under heat and salinity treatments, respectively. Meanwhile, expression of TtNAMB-2 was significantly induced by salt and low-temperature stresses. Salt treatment induced expression of TtNAMB-2 and caused a 13-fold increase in transcript copy numbers at 48 h posttreatment. Examination of expression changes under abiotic stresses may provide important information for understanding roles of TaNAC69-1 and TtNAMB-2 genes which might be involved in response to environmental stresses. © 2012 Springer-Verlag.
Anahtar Kelimeler
Gene expression | NAC-type transcription factors | RT-qPCR | Wheat
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı PLANT MOLECULAR BIOLOGY REPORTER
Dergi ISSN 0735-9640
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 10-2012
Cilt No 30
Sayı 5
Sayfalar 1246 / 1252
Doi Numarası 10.1007/s11105-012-0445-3
Makale Linki http://link.springer.com/article/10.1007%2Fs11105-012-0445-3