| Yazarlar |
Prof. Dr. Mehmet Cengiz BALOĞLU
Kastamonu Üniversitesi, Türkiye |
Abdulhamit Battal
Yüzüncü Yıl Üniversitesi, Türkiye |
|
Gülsüm Aydın
Selçuk Üniversitesi, Türkiye |
|
Ayten Eroğlu
Yüzüncü Yıl Üniversitesi, Türkiye |
|
Öz Mehmet Tufan
|
|
Musa Kavas
Ondokuz Mayıs Üniversitesi, Türkiye |
|
Hüseyin Avni Öktem
Konya Gıda ve Tarım Üniversitesi, Türkiye |
|
Ayşe Meral Yücel
Orta Doğu Teknik Üniversitesi, Türkiye |
| Özet |
| Gene cloning and vector construction for plant genetic transformation are routine procedures in plant functional genomic studies. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. Here, we describe the construction of a series of transformation vectors through different genetic engineering techniques for wheat transformation. For this purpose, NAC-type transcription factor genes TaNAC69-1andTtNAM-B2 were isolated from T. aestivum and T. turgidum, respectively. Then they were cloned into different types of cloning and expression vectors. Besides traditional restriction enzyme digestion and ligation method, Gateway cloning technology which is a fast and reliable alternative cloning method were used for construction of wheat transformation vectors. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium based transformation protocols for wheat plant. |
| Anahtar Kelimeler |
| Gatewaygene cloning | Traditional gene cloning | Vector construction | Wheat transformation |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF ANIMAL AND PLANT SCIENCES |
| Dergi ISSN | 1018-7081 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2013 |
| Cilt No | 23 |
| Sayı | 3 |
| Sayfalar | 906 / 912 |
| Makale Linki | http://www.thejaps.org.pk/Volume/2013/23-3/default.asp |
