Molecular Characterization 3D Model Analysis and Expression Pattern of the CmUBC Gene Encoding the Melon Ubiquitin Conjugating Enzyme Under Drought and Salt Stress Conditions
 
Yazarlar (2)
Prof. Dr. Mehmet Cengiz BALOĞLU Kastamonu Üniversitesi, Türkiye
Meliha Gorkem Patir Universität Heidelberg, Almanya
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Biochemical Genetics
Dergi ISSN 0006-2928 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 02-2014
Kabul Tarihi Yayınlanma Tarihi 10-11-2013
Cilt / Sayı / Sayfa 52 / 1 / 90–105 DOI 10.1007/s10528-013-9630-9
Makale Linki http://link.springer.com/article/10.1007%2Fs10528-013-9630-9
UAK Araştırma Alanları
Biyoteknoloji
Özet
Ubiquitin-conjugating (UBC) enzyme is a key enzyme in ubiquitination. Here, we describe the cloning, characterization, and expression pattern of a novel gene, CmUBC, from a melon. Comparison of the deduced amino acid sequences allowed the identification of highly conserved motifs. Synteny analysis between Cucumis sativus L. and Arabidopsis demonstrated that homologs of several Cucumis UBC genes were found in corresponding syntenic blocks of Arabidopsis. The homology structure model of the CmUBC protein was constructed. UBCs from melon, yeast, and Arabidopsis were highly conserved in their three-dimensional folding. CmUBC was ubiquitously expressed in all melon tissues. Increased transcript levels of CmUBC were observed during drought and salinity stresses, which suggested that the expression of the CmUBC gene in melon plants is responsive to physiological water stress. These …
Anahtar Kelimeler
3D model analysis | Cucumis melo L. | Gene expression | Ubiquitin-conjugating enzyme (UBC) | Water-stress responses