Optimization of yeast Saccharomyces cerevisiae RNA isolation method for real time quantitative PCR and microarray analysis
Yazarlar (6)
Remziye Yilmaz
Oya Akca
Mehmet Cengiz Baloglu
Mehmet Tufan Oz
Huseyin Avni Oktem
Meral Yucel
| Makale Türü |
Özgün Makale
(SCOPUS dergilerinde yayınlanan tam makale)
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| Dergi Adı | African Journal of Biotechnology | ||
| Dergi ISSN | 1684-5315 | ||
| Dergi Tarandığı Indeksler | Scopus | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2012 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 16-01-2012 |
| Cilt / Sayı / Sayfa | 11 / 5 / 1046–1053 | DOI | 10.5897/AJB11.2994 |
| Makale Linki | http://www.academicjournals.org/journal/AJB/edition/16_January_2012 | ||
| UAK Araştırma Alanları |
Biyoteknoloji
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| Özet |
| Quality of the starting RNA is indispensably important for obtaining highly reproducible quantitative polymerase chain reaction (qPCR) and microarray results for all organisms as well as S. cerevisiae. Isolating RNA from yeast cells with a maximum quality was especially critical since these cells were rich in polysaccharides and proteins. The method has been optimized through modification pretreatment applications for the isolation of S. cerevisiae RNA for qPCR and microarray analysis. Two extraction assay a TRIzol reagent-based method with three pretreatment applications and a commercially available kit with own pretreatment application, were compared for this purpose. Furthermore, the concentration yeast cells and enzyme were controlled in the range of 2 × 106 to 6 × 107 cells and 0.5 to 5 mg/ml, respectively to prevent RNA yields decrease and RNA degradation. Results of RNA isolation of the middle … |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
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| Google Scholar | 12 |