| Yazarlar |
Latife Betül Gül
Giresun Üniversitesi, Türkiye |
|
Ahmet Hilmi Çon
Ondokuz Mayıs Üniversitesi, Türkiye |
Prof. Dr. Osman GÜL
Kastamonu Üniversitesi, Türkiye |
| Özet |
| In this study, the response surface methodology was used to optimize the cryoprotective agent (skimmed milk powder, lactose and sucrose) formulation for enhancing the viability of Lactobacillus curvatus N19 during freeze-drying and storage stability of cells freeze-dried by using optimum formulation was evaluated. Our results showed that the most significant cryoprotective agent influencing the viability of L. curvatus N19 to freezing and freeze-drying was sucrose and skim milk, respectively. The optimal formulation of cryoprotective agents was 20 g/100 mL skim milk, 3.57 g/100 mL lactose and 10 g/100 mL sucrose. Using the optimum formulation during freeze-drying, the cell survival was found more than 98%. Under the optimal conditions, although only storage of the cells at 4 °C for 6 month retained the maximum stability (8.85 log cfu/g), the employed protectant matrix showed promising results at 25 °C (7.89 log cfu/g). The storage stability of cells under optimized conditions was predicted by accelerated storage test, which was demonstrated that the inactivation rate constant of the freeze-dried L. curvatus N19 powder was 9.74 × 10−6 1/d for 4 °C and 2.08 × 10−3 1/d for 25 °C. The loss of specific acidification activity after the storage at 4 and 25 °C was determined. |
| Anahtar Kelimeler |
| Acidification activity | Cryoprotectant | Freeze-drying | Lactobacillus curvatus N19 | Response surface method | Storage stability |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | CRYOBIOLOGY |
| Dergi ISSN | 0011-2240 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 10-2020 |
| Cilt No | 96 |
| Sayı | 1 |
| Sayfalar | 122 / 129 |
| Doi Numarası | 10.1016/j.cryobiol.2020.07.007 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0011224020302479 |
| Atıf Sayıları | |
| SCOPUS | 30 |
| Google Scholar | 34 |
