| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Cryobiology | ||
| Dergi ISSN | 0011-2240 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 10-2020 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-10-2020 |
| Cilt / Sayı / Sayfa | 96 / 1 / 122–129 | DOI | 10.1016/j.cryobiol.2020.07.007 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0011224020302479 | ||
| Özet |
| In this study, the response surface methodology was used to optimize the cryoprotective agent (skimmed milk powder, lactose and sucrose) formulation for enhancing the viability of Lactobacillus curvatus N19 during freeze-drying and storage stability of cells freeze-dried by using optimum formulation was evaluated. Our results showed that the most significant cryoprotective agent influencing the viability of L. curvatus N19 to freezing and freeze-drying was sucrose and skim milk, respectively. The optimal formulation of cryoprotective agents was 20 g/100 mL skim milk, 3.57 g/100 mL lactose and 10 g/100 mL sucrose. Using the optimum formulation during freeze-drying, the cell survival was found more than 98%. Under the optimal conditions, although only storage of the cells at 4 °C for 6 month retained the maximum stability (8.85 log cfu/g), the employed protectant matrix showed promising results at 25 °C (7.89 log … |
| Anahtar Kelimeler |
| Acidification activity | Cryoprotectant | Freeze-drying | Lactobacillus curvatus N19 | Response surface method | Storage stability |
| Atıf Sayıları | |
| Scopus | 40 |
| Google Scholar | 43 |
| Dergi Adı | CRYOBIOLOGY |
| Yayıncı | Academic Press Inc. |
| Açık Erişim | Hayır |
| ISSN | 0011-2240 |
| E-ISSN | 1090-2392 |
| CiteScore | 4,4 |
| SJR | 0,519 |
| SNIP | 0,915 |