Optimization of cryoprotectant formulation to enhance the viability of Lactobacillus brevis ED25: Determination of storage stability and acidification kinetics in sourdough
Yazarlar (5)
Dr. Öğr. Üyesi Latife Betül Gül Giresun Üniversitesi, Türkiye
Prof. Dr. Osman GÜL Kastamonu Üniversitesi, Türkiye
Mustafa Tahsin Yılmaz Yıldız Teknik Üniversitesi, Türkiye
Enes Dertli Yıldız Teknik Üniversitesi, Türkiye
Prof. Dr. Ahmet Hilmi Çon Ondokuz Mayıs Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Journal of Food Processing and Preservation
Dergi ISSN 0145-8892 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 04-2020
Kabul Tarihi 02-02-2020 Yayınlanma Tarihi 17-02-2020
Cilt / Sayı / Sayfa 44 / 4 / – DOI 10.1111/jfpp.14400
Makale Linki https://onlinelibrary.wiley.com/doi/abs/10.1111/jfpp.14400
Özet
In this study, various kinds of cryoprotectant (skim milk, lactose, and sucrose) formulations were tested to enhance the survival of Lactobacillus brevis ED25 after freezing and freeze‐drying. A Box–Behnken experimental design was used to optimize cryoprotective medium and the highest cell survival was observed with the 17.28% skim milk, 2.12% lactose, and 10% sucrose cryoprotectant as the optimum condition. The structural and physicochemical characteristics of freeze‐dried powder were acceptable for application with regards to particle surface morphology, moisture and water activity (Aw), glass transition temperature (Tg), Fourier transform infrared spectra, X‐ray structure, and also storage stability under the refrigeration and room temperature conditions. Accelerated storage test based on Arrhenius equation could be used to predict the freeze‐dried bacterial shelf life but only with a certain degree of …
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