| Yazarlar |
Fadime Mutlu İçduygu
Giresun Üniversitesi, Türkiye |
|
Hale Şamlı
Bursa Uludağ Üniversitesi, Türkiye |
Doç. Dr. Asuman ÖZGÖZ
Kastamonu Üniversitesi, Türkiye |
|
Buse Vatansever
|
|
Kuyaş Hekimler Öztürk
Süleyman Demirel Üniversitesi, Türkiye |
|
Egemen Akgün
Giresun Üniversitesi, Türkiye |
| Özet |
| Background. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells. |
| Anahtar Kelimeler |
| Cancer stem cell | Paclitaxel | Prostate cancer | Taxane resistance |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Advances in Clinical and Experimental Medicine |
| Dergi ISSN | 1899-5276 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2021 |
| Cilt No | 30 |
| Sayı | 12 |
| Sayfalar | 1283 / 1291 |
| Doi Numarası | 10.17219/acem/140590 |
| Atıf Sayıları | |
| SCOPUS | 6 |
