Possibility of paclitaxel to induce the stemness-related characteristics of prostate cancer cells
Yazarlar (6)
Doç. Dr. Fadime Mutlu İçduygu Giresun Üniversitesi, Türkiye
Prof. Dr. Hale Şamlı Bursa Uludağ Üniversitesi, Türkiye
Doç. Dr. Asuman ÖZGÖZ Kastamonu Üniversitesi, Türkiye
Öğr. Gör. Buse Vatansever Bursa Uludağ Üniversitesi, Türkiye
Doç. Dr. Kuyaş Hekimler Öztürk Süleyman Demirel Üniversitesi, Türkiye
Dr. Öğr. Üyesi Egemen Akgün Giresun Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Advances in Clinical and Experimental Medicine (Q4) | ||
| Dergi ISSN | 1899-5276 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2021 |
| Cilt / Sayı / Sayfa | 30 / 12 / 1283–1291 | DOI | 10.17219/acem/140590 |
| Makale Linki | https://advances.umw.edu.pl/pdf/2021/30/12/1283.pdf | ||
| UAK Araştırma Alanları |
Tıbbi Genetik
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| Özet |
| Background. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells. |
| Anahtar Kelimeler |
| Cancer stem cell | Paclitaxel | Prostate cancer | Taxane resistance |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 8 |