Isolation and Characterization of Lytic Bacteriophages from Wastewater with Phage Therapy Potentials Against Gram-Negative Bacteria
Yazarlar (6)
Mona Khorshidtalab
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
İnci Durukan
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
Dr. Öğr. Üyesi Enis Fuat TÜFEKCİ Kastamonu Üniversitesi, Türkiye
Seyran S. Nas
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
Mujib A. Abdurrahman
Dire Dawa University, Etiyopya
Dire Dawa University, Etiyopya
Ali O. Kiliç
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
Karadeniz Teknik Üniversitesi Tip Fakültesi, Türkiye
| Makale Türü |
Özgün Makale
(ESCI dergilerinde yayınlanan tam makale)
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| Dergi Adı | Eurasian Journal of Medicine | ||
| Dergi ISSN | 1308-8734 | ||
| Dergi Tarandığı Indeksler | ESCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2022 |
| Cilt / Sayı / Sayfa | 54 / 2 / 157–164 | DOI | 10.5152/eurasianjmed.2022.21010 |
| Makale Linki | http://dx.doi.org/10.5152/eurasianjmed.2022.21010 | ||
| UAK Araştırma Alanları |
Tıbbi Mikrobiyoloji
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| Özet |
| Objective The increase of multidrug resistance in bacteria has increased the efforts in search of alternative methods. The aim of the present study was to isolate and characterize the lytic phages and assess their lytic activity against a number of gram-negative bacteria. Materials and Methods The phages and their respective hosts were isolated from wastewater collected from the municipal sewer system of Trabzon, Turkey. The lytic activities of phage were determined using the agar spot test. The identification and antibiotic susceptibility of host bacteria were determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry and Phoenix 100, respectively. The phages were characterized morphologically using transmission electron microscopy. One of the phages, Enteroc21, which has a broad-host-range, was further characterized by genome restriction endonuclease analysis and burst … |
| Anahtar Kelimeler |
| Bacteriophages | multidrug resistance | Myoviridae | Podoviridae | Siphoviridae |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 13 |
| Google Scholar | 24 |