Reduced lecithin Cholesterol acyltransferase LCAT and Na K ATPase activity in diabetic patients
Yazarlar (5)
Prof. Dr. Ahmet Kızıltunç Atatürk Üniversitesi, Türkiye
Doç. Dr. Fatih Akçay Atatürk Üniversitesi, Türkiye
Sevinç Kuşkay Kocaeli Üniversitesi, Türkiye
Muhammet Fevzi Polat Bozok Üniversitesi, Türkiye
Yaşar Nuri Şahin
Atatürk Üniversitesi, Türkiye
Atatürk Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Clinical Biochemistry | ||
| Dergi ISSN | 0009-9120 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-1997 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-03-1997 |
| Cilt / Sayı / Sayfa | 30 / 2 / 177–182 | DOI | 10.1016/S0009-9120(96)00156-7 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0009912096001567 | ||
| UAK Araştırma Alanları |
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| Özet |
| Objectives: To investigate the plasma concentrations of lecithin:cholesterol acyltransferase (LCAT EC 2.3.1.43) and erythrocyte membrane Na+,K+,ATPase and the correlation of these parameters in diabetes mellitus. Design and Methods: Na+,K+,ATPase was measured with spectrophotometric method and LCAT with radioactive method in 19 patients with insulin-dependent diabetes mellitus (IDDM), in 20 with non-insulin- dependent diabetes mellitus (NIDDM) and in 20 healthy volunteers as the control group. Results: Compared with the control group, plasma LCAT concentrations were found to be decreased in both of the patient groups (p < 0.01 for both). Erythrocyte membrane Na+,K+,ATPase activities were higher in the controls than both in the NIDDM and IDDM groups (p < 0.01 and p < 0.001, respectively). There were significant correlations between LCAT and Na+,K+,ATPase in IDDM (r = 0.82, p < 0.001) and in NIDDM (r= 0.74, p < 0.001). In order to investigate the effect of cholesterol (C) and lysophosphatidylcholine (LPC) on Na+,K+,ATPase activity, this enzyme's activity was determined in enythrocyte membranes obtained from diabetic subjects after in vitro incubation with increasing concentrations of LPC and C (2-10 μM). Enzymatic activity was significantly reduced by in vitro C at increasing concentrations but significantly increased by in vitro LPC at increasing concentrations. Conclusions: From these data, it is to be concluded that the decrease in Na+,K+,ATPase activity in diabetes might be due to decreased LCAT concentrations and that may explain the development of atherosclerosis in diabetics. |
| Anahtar Kelimeler |
| ATPase | cholesterol acyltransferase | diabetes mellitus | K + | lecithin | Na + |
BM Sürdürülebilir Kalkınma Amaçları
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