| Yazarlar |
Ahmet Kızıltunç
Atatürk Üniversitesi, Türkiye |
|
Fatih Akçay
Atatürk Üniversitesi, Türkiye |
|
Sevinç Kuşkay
Kocaeli Üniversitesi, Türkiye |
|
Muhammet Fevzi Polat
Bozok Üniversitesi, Türkiye |
Prof. Dr. Yaşar Nuri ŞAHİN
Atatürk Üniversitesi, Türkiye |
| Özet |
| Objectives: To investigate the plasma concentrations of lecithin:cholesterol acyltransferase (LCAT EC 2.3.1.43) and erythrocyte membrane Na+,K+,ATPase and the correlation of these parameters in diabetes mellitus. Design and Methods: Na+,K+,ATPase was measured with spectrophotometric method and LCAT with radioactive method in 19 patients with insulin-dependent diabetes mellitus (IDDM), in 20 with non-insulin- dependent diabetes mellitus (NIDDM) and in 20 healthy volunteers as the control group. Results: Compared with the control group, plasma LCAT concentrations were found to be decreased in both of the patient groups (p < 0.01 for both). Erythrocyte membrane Na+,K+,ATPase activities were higher in the controls than both in the NIDDM and IDDM groups (p < 0.01 and p < 0.001, respectively). There were significant correlations between LCAT and Na+,K+,ATPase in IDDM (r = 0.82, p < 0.001) and in NIDDM (r= 0.74, p < 0.001). In order to investigate the effect of cholesterol (C) and lysophosphatidylcholine (LPC) on Na+,K+,ATPase activity, this enzyme's activity was determined in enythrocyte membranes obtained from diabetic subjects after in vitro incubation with increasing concentrations of LPC and C (2-10 μM). Enzymatic activity was significantly reduced by in vitro C at increasing concentrations but significantly increased by in vitro LPC at increasing concentrations. Conclusions: From these data, it is to be concluded that the decrease in Na+,K+,ATPase activity in diabetes might be due to decreased LCAT concentrations and that may explain the development of atherosclerosis in diabetics. |
| Anahtar Kelimeler |
| ATPase | cholesterol acyltransferase | diabetes mellitus | K + | lecithin | Na + |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Clinical Biochemistry |
| Dergi ISSN | 0009-9120 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-1997 |
| Cilt No | 30 |
| Sayı | 2 |
| Sayfalar | 177 / 182 |
| Doi Numarası | 10.1016/S0009-9120(96)00156-7 |
| Atıf Sayıları | |
| SCOPUS | 30 |
| Google Scholar | 55 |
