| Yazarlar |
Murat Çetin Rağbetli
Van Yüzüncü Yıl Üniversitesi, Türkiye |
|
Mikail Kara
Hatay Mustafa Kemal Üniversitesi, Türkiye |
|
Nese Çölçimen
|
|
Necat Koyun
Van Yüzüncü Yıl Üniversitesi, Türkiye |
|
Gamze Çakmak
Van Yüzüncü Yıl Üniversitesi, Türkiye |
|
Veysel Akyol
Van Yüzüncü Yıl Üniversitesi, Türkiye |
|
Ömür Gülsüm Deniz
Türkiye |
Doç. Dr. Kıymet Kübra TÜFEKCİ
Kastamonu Üniversitesi, Türkiye |
| Özet |
| The main purpose of the present study was to investigate the effects of diclofenac sodium (DS) on the total number of neurons in segment T13 of the spinal cord of offspring of pregnant rats using stereological methods. Eighteen adult female Wistar albino rats weighing 150–200 g were used. Pregnant female rats were divided into three groups; a control group, a sham group and a DS (1 mg/kg, intramuscular) exposed group. The DS and sham groups received injection from the 5th day of gestation to the 19th. Twenty eight days after birth, the offspring rats were perfused with 4% buffered formalin. T13, which is one of transverse spinal cord segments, were isolated and processed for routine paraffin histology. 5 μm sections were obtained using a rotary microtome according to systematic random sampling strategies. Every 40th section was taken and sections were stained with modified Giemsa. All types of motor neuron cell were identified according to their morphology. In this study, the “disector-Cavalieri combination” method was used in the stereological examination of neurons. The motor neurons were counted in the right gray matter of the ventral horn in the spinal cord segment. The Kruskal–Wallis test was used for comparison the groups. In terms of motoneuron number, no significant difference among the groups was found (p > 0.05). In conclusion, our results indicated that prenatal exposure to DS has no effect on the total number of motor neuron of the offspring rats. |
| Anahtar Kelimeler |
| Diclofenac sodium,Motoneurons,Prenatal,Rat,Spinal segment T13,Stereology |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF CHEMICAL NEUROANATOMY |
| Dergi ISSN | 0891-0618 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2018 |
| Cilt No | 87 |
| Sayfalar | 49 / 53 |
| Doi Numarası | 10.1016/j.jchemneu.2017.05.001 |
| Makale Linki | http://dx.doi.org/10.1016/j.jchemneu.2017.05.001 |
| Atıf Sayıları | |
| WoS | 1 |
| SCOPUS | 2 |
