Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration
Yazarlar (3)
Doç. Dr. Büşra Dincer Ondokuz Mayıs Üniversitesi, Türkiye
Azad Çekdar Gundogmus
Doç. Dr. İrfan ÇINAR Kastamonu Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale)
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| Dergi Adı | Experimental Biomedical Research | ||
| Dergi ISSN | 2618-6454 | ||
| Dergi Tarandığı Indeksler | TR DİZİN | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2023 |
| Cilt / Sayı / Sayfa | 6 / 3 / 238–248 | DOI | 10.30714/j-ebr.2023.188 |
| Makale Linki | https://experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/download/380/196/ | ||
| UAK Araştırma Alanları |
Tıbbi Farmakoloji
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| Özet |
| Aim: Oxidative stress has been a significant factor in wound-healing pathophysiology for a long time. Antioxidants, especially natural compounds, have recently been emphasized in instructions for wound healing treatments. Jaceosidin (JACE), a flavone derived from Artemisia princeps, is a potent antioxidant. This study aims to investigate JACE’s anti-inflammatory and antioxidant properties and its capacity to improve the effects of in vitro wound healing.Methods: Wound healing activities have been tested using cell proliferation and migration in vitro assays in the mouse fibroblast cell line L929. The concentration of hydrogen peroxide (H2O2-0.5 mM) has been used to induce the oxidative stress model. Tumor necrosis factor-alpha (TNF-α) and nuclear factor (NF-κβ) have been investigated as inflammatory indicators. Antioxidant activity has been checked using total antioxidant status (TAS) and total oxidant status (TOS) tests.Results: JACE has significantly increased the proliferation of fibroblasts dose-dependent manner. It has enhanced the cell migration rate of fibroblasts compared with the H2O2 group. JACE at a concentration of 50 and 100 μM has significantly decreased TOS and oxidative stress index (OSI) levels and increased TAS levels. The anti-inflammatory mechanism of JACE has involved down-regulation of the mRNA expressions of the NF-κβ and TNF-α in a dose-dependent manner.Conclusions: JACE has beneficial impacts on fibroblast viability and migration qualities through antioxidative actions and down-regulating proinflammatory cytokines through anti-inflammatory effects to promote wound healing. The present study shows … |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
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