| Yazarlar |
Murat Karameşe
Kafkas Üniversitesi, Türkiye |
|
Bülent Güvendi
Recep Tayyip Erdoğan Üniversitesi, Türkiye |
|
Selina Aksak Karameşe
Kafkas Üniversitesi, Türkiye |
Doç. Dr. İrfan ÇINAR
Kafkas Üniversitesi, Türkiye |
|
Serpil Can
Kafkas Üniversitesi, Türkiye |
Doç. Dr. Hüseyin Serkan EROL
Atatürk Üniversitesi, Türkiye |
|
Hakan Aydın
Atatürk Üniversitesi, Türkiye |
|
Volkan Gelen
Kafkas Üniversitesi, Türkiye |
|
Emre Karakuş
Türkiye |
| Özet |
| Objective(s): In the present study, our aim was to investigate the possible protective effects of epigallocatechin gallate (EGCG) on lipopolysaccharide (LPS)-induced hepatotoxicity by using Hep3B human hepatoma cells. Specifically, the study examines the role of some proinflammatory markers and oxidative damage as possible mechanisms of LPS-associated cytotoxicity. Consequently, the hepatocellular carcinoma cell line Hep3B was chosen as a model for investigation of LPS toxicity and the effect of EGCG on LPS-exposed cells. Materials and Methods: The Hep3B human hepatoma cells were used for this study. The cytotoxic effects of chemicals (EGCG and LPS), AST and ALT levels, SOD and CAT activities, GSH-Px level and TNF-alpha and IL-6 levels were detected by using different biochemical and molecular methods. LPS and EGCG were applied to cells at various times and doses. Results: The highest treatment dose of EGCG (400 µM) led to a dramatic decrease in SOD level and increase in CAT and GSH levels. Additionally, the highest dose of EGCG also led to a dramatic increase in TNF-alpha and IL-6 levels. On the other hand, effective doses of EGCG (200 and 100 µM) normalized all related parameters levels. Conclusion: LPS caused hepatotoxicity, but interestingly, a high dose of EGCG was found to be a cytotoxic agent in this study. However, other two doses of EGCG led to a decrease in both inflammatory cytokine levels and antioxidant enzyme levels. Further studies should examine the effect of EGCG on secondary cellular signaling pathways. |
| Anahtar Kelimeler |
| Cytokines | Epigallocatechin gallate | Hep3B | Lipopolysaccharide | Oxidative stress |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES |
| Dergi ISSN | 2008-3866 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2016 |
| Cilt No | 19 |
| Sayı | 5 |
| Sayfalar | 483 / 489 |
