| Yazarlar (1) |
Doç. Dr. İrfan ÇINAR
Kastamonu Üniversitesi, Türkiye |
| Özet |
| Aims: The rapid growth, morbidity and mortality of lung cancer and the lack of effective treatment have attracted great interest from researchers to find new cancer treatments aimed at the effect of gossipine on cell proliferation and apoptosis of A549 cells. The most used of these products are flavonoids. Gossypin is a potential chemopreventive and therapeutic agent for lung cancer.Methods: We investigated the effect of Gossypin anticancer activity on A549 cell proliferation with MTT method, depending on dose and time. In addition, mRNA expression levels of the apoptotic markers caspase-3 (CAS-3) and caspase-9 (CAS-9) were investigated by real-time PCR. In our study, six groups were used as control, gossypinin (10, 20, 40 µM), cisplatin 5 µg/mL and cisplatin 5 µg/mL+ gossypine 40 µM combine concentrations. The proliferative and antiapoptotic effects of gossypin at 24, 48 and 72 hours were investigated. Results were analyzed and presented as cell viability (%).Results: In our results, it was determined that gossypin especially at a dose of 40 µM and at 72 hours showed almost as much effect on A549 cells, but the highest inhibitory effect was seen in the 40 combined group of cisplatin 5 µg/mL+ gossypine. In addition, gossypin caused a significant increase in apoptosis genes (CASP-3, CASP-9) compared to control.Conclusion: Our study showed that gossypin significantly increases the chemosensitivity of cisplatin. Based on this, it is predicted that gossypine can be used as a supportive therapy that increases the effectiveness of anticancer agents. However, more detailed research should be done for this. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Diğer hakemli ulusal dergilerde yayınlanan tam makale |
| Dergi Adı | KASTAMONU MEDICAL JOURNAL |
| Dergi ISSN | 2757-9336 |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2021 |
| Cilt No | 1 |
| Sayı | 2 |
| Sayfalar | 37 / 40 |
| Doi Numarası | 10.51271/KMJ-0009 |
| Makale Linki | https://kastamonutipdergisi.com/makale-detay.php?id=12 |
| Atıf Sayıları |
