Biochemical Investigation of the Protective Effect of Thiamine Pyrophosphate on Methotrexate-Induced Gastrotoxicity in Rats
Yazarlar (3)
Doç. Dr. İrfan ÇINAR Atatürk Üniversitesi, Türkiye
Mine Gulapoglu
Atatürk Üniversitesi, Türkiye
Atatürk Üniversitesi, Türkiye
Prof. Dr. Halis Suleyman Faculty Of Medicine, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Indian Journal of Pharmaceutical Education and Research | ||
| Dergi ISSN | 0019-5464 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2020 |
| Cilt / Sayı / Sayfa | 54 / 2 / 397–402 | DOI | 10.5530/ijper.54.2.45 |
| Makale Linki | http://ijper.org/article/1146 | ||
| Özet |
| Aim The aim of the study is to investigate the protective effect of thiamine pyrophosphate on methotrexate-induced gastrotoxicity biochemically in rats and to compare it with thiamine. Methods Rats were divided into four groups as sham (SG), thiamine+ methotrexate (TAMTX), thiamine pyrophosphate+ methotrexate (TPMTX) and Methotrexate (MTX) groups. 5 mg/kg methotrexate was administrated to 24 hrfasted rat groups by oral gastric tube except sham rats. 20 mg/kg thiamine was injected to TAMTX group and 20 mg/kg thiamine pyrophosphate was injected to TPMTX group ip 5 min before the methotrexate administration. Equal amount distilled water was injected ip to SG and MTX groups. 6 hr after the methotrexate administration, the rats were sacrificed with high dose thiopental anesthesia (50 mg/kg). The stomachs of the rats were extracted and MDA, MPO, GSH and SOD analyzes were performed. Results Biochemical results were evaluated by comparing among groups. While tGSH and MDA levels and MPO and SOD activities were 4.2±0.6, 10.0±0.9, 7.4±0.8, 5.2±0.9 µmol/g in MTX group, these parameters were 11.0±1.0, 3.4±0.4, 3.3±0.5, 13.2±1.1 µmol/g in SG group in the gastric tissue. These parameters were measured as 4.8±0.8, 8.7±0.8, 5.9±0.5, 6.1±0.8 µmol/g in TAMTX group and 10.8±0.8, 4.0±0.5, 3.6±0.4, 10.5±0.9 µmol/g in TPMTX group. Conclusion The results showed that thiamine pyrophosphate prevented methotrexate induced oxidative damage in the gastric tissue, but thiamine could not prevent. |
| Anahtar Kelimeler |
| Gastrotoxicity | Methotrexate | Thiamin pyrophosphate | Thiamine | Ulcer |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 2 |
| Scopus | 3 |
| Google Scholar | 3 |