| Yazarlar |
Şaziye Sezin Palabıyık
Atatürk Üniversitesi, Türkiye |
|
Emre Karakuş
Türkiye |
|
Zekai Halıcı
Atatürk Üniversitesi, Türkiye |
|
Elif Çadırcı
Atatürk Üniversitesi, Türkiye |
|
Yasin Bayır
Atatürk Üniversitesi, Türkiye |
|
Gülşen Ayaz
|
Doç. Dr. İrfan ÇINAR
Kafkas Üniversitesi, Türkiye |
| Özet |
| Acetaminophen (APAP) overdose could induce liver damage and lead to acute liver failure. The treatment of APAP overdoses could be improved by new therapeutic strategies. Thymus spp., which has many beneficial effects and has been used in folk medicine, is one such potential strategy. In the present study, the hepatoprotective activity of the main constituents of Thymus spp., carvacrol and thymol, were evaluated in light of APAP-induced hepatotoxicity. We hoped to understand the hepatoprotective mechanism of these agents on the antioxidant system and pro-inflammatory cytokines in vitro. Dose-dependent effects of thymol and carvacrol (25, 50, and 100 μM) were tested on cultured HepG2 cells. N-Acetylcysteine (NAC) was tested as positive control. We showed that APAP inhibited HepG2 cell growth by inducing inflammation and oxidative stress. Incubating APAP-exposed HepG2 cells with carvacrol and thymol for 24 h ameliorated this inflammation and oxidative stress. We also evaluated alanine transaminase and lactate dehydrogenase levels of HepG2 cells. We found that thymol and carvacrol protected against APAP-induced toxicity in HepG2 cells by increasing antioxidant activity and reducing pro-inflammatory cytokines, such as tumor necrosis factor α and interleukin 1β. Taking together high-dose thymol and carvacrol treatment has an effect close to NAC treatment in APAP toxicity, but thymol has better treatment effect than carvacrol. |
| Anahtar Kelimeler |
| Acetaminophen toxicity | carvacrol | HepG2-cultured cell | IL-1β | thymol | TNF-α |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | HUMAN EXPERIMENTAL TOXICOLOGY |
| Dergi ISSN | 0960-3271 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2016 |
| Cilt No | 35 |
| Sayı | 12 |
| Sayfalar | 1252 / 1263 |
| Doi Numarası | 10.1177/0960327115627688 |
| Atıf Sayıları | |
| SCOPUS | 57 |
| Google Scholar | 74 |
