Effects of using collagen and aloe vera grafted fibroin scaffolds on osteogenic differentiation of rat bone marrow mesenchymal stem cells in SBF-enriched cell culture medium
Yazarlar (2)
Salma A. Taher Mohamed Kastamonu Üniversitesi, Türkiye
Doç. Dr. Nuray EMİN Kastamonu Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Biomedical Materials Bristol (Q2) | ||
| Dergi ISSN | 1748-6041 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2024 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 14-12-2023 |
| Cilt / Sayı / Sayfa | 19 / 1 / 15011–0 | DOI | 10.1088/1748-605X/ad12e2 |
| Makale Linki | https://doi.org/10.1088/1748-605x/ad12e2 | ||
| Özet |
| In the study, collagen and aloe vera were grafted onto silk fibroin with two different methods, and 3D-microporous scaffolds (1F5C4A1 and 2F5C4A1) were formed by lyophilization. Three osteogenic cultures were started by seeding rat bone marrow mesenchymal stem cells (MSCs) and pre-induced MSC (osteoblast (OB)) on biopolymeric scaffolds. The osteogenic medium was enriched with 10% (v/v) simulated body fluid (SBF) to promote mineralization and osteogenic differentiation in one of the MSC cultures and the OB culture. X-ray diffraction (XRD), scanning electron microscopy (SEM), scanning electron microscopy- energy dispersive spectrum (SEM-EDS) analyses on cellular samples and histochemical (alizarin red, safranin-O, alcian blue) and immunohistochemical (anti-collagen-1, anti-osteocalcin, anti-osteopontin) staining showed that bone-like mineralization was occurred by both chemically and cellular … |
| Anahtar Kelimeler |
| aloe vera | biopolymeric scaffold | bone tissue engineering | collagen | fibroin | MSC | SBF |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 6 |
| Scopus | 7 |
| Google Scholar | 7 |