Engineering of Rat Articular Cartilage on Porous Sponges: Effects of TGF-β 1and Microgravity Bioreactor Culture
Yazarlar (5)
Doç. Dr. Nuray EMİN Ankara Üniversitesi, Türkiye
Aysel Koç Ankara Üniversitesi, Türkiye
Serap Durkut Ankara Üniversitesi, Türkiye
A. Eser Elçin Ankara Üniversitesi, Türkiye
Y. Murat Elçin Ankara Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Artificial Cells Blood Substitutes and Biotechnology (Q4) | ||
| Dergi ISSN | 1073-1199 | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2008 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-01-2008 |
| Cilt / Sayı / Sayfa | 36 / 2 / 123–137 | DOI | 10.1080/10731190801932116 |
| Makale Linki | http://dx.doi.org/10.1080/10731190801932116 | ||
| Özet |
| The objective of this study was to develop an engineered rat hyaline cartilage by culturing articular chondrocytes on three-dimensional (3D) macroporous poly(DL-lactic-co-glycolic acid) (PLGA) sponges under chondrogenic induction and microgravity bioreactor conditions. Experimental groups consisted of 3D static and dynamic cultures, while a single cell monolayer (2D) served as the control. The effect of seeding conditions (static vs. dynamic) on cellularization of the scaffolds was investigated. MTT assay was used to evaluate the number of viable cells in each group at different time points. Formation of a hyaline-like cartilage was evaluated for up to 4 weeks in vitro. While 2D culture resulted in cell sheets with very poor matrix production, 3D culture was in the favor of tissue formation. A higher yield of cell attachment and spatially uniform cell distribution was achieved when dynamic seeding technique was used … |
| Anahtar Kelimeler |
| Articular cartilage | Chondrogenesis | Microgravity bioreactor | PLGA sponge | Rat | TGF-β 1 | Tissue engineering |
Science Direct
Engineering of Rat Articular Cartilage on Porous Sponges: Effects of TGF-β 1and Microgravity Bioreactor Culture
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 40 |
| Scopus | 47 |
| Google Scholar | 74 |