Effect of dexamethasone on unfolded protein response genes (MTJ1, Grp78, Grp94, CHOP, HMOX-1) in HEp2 cell line
Yazarlar (8)
Aynur Düzgün Ankara Branch Of Council Of Forensic Medicine, Türkiye
Abdülkerim Bedir
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
Tülay Özdemir Gazi State Hospital, Samsun, Türkiye
Rukiye Nar
Pamukkale Üniversitesi, Türkiye
Pamukkale Üniversitesi, Türkiye
Veli Kılınç
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
Osman Saliş Mental Health And Diseases Hospital, Türkiye
Hasan Alaçam Ondokuz Mayis Üniversitesi, Türkiye
Doç. Dr. Sedat GÜLTEN Kastamonu Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Indian Journal of Biochemistry and Biophysics | ||
| Dergi ISSN | 0301-1208 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2013 |
| Cilt / Sayı / Sayfa | 50 / 6 / 505–510 | DOI | – |
| Makale Linki | https://nopr.niscair.res.in/handle/123456789/25168 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| The endoplasmic reticulum (ER) is related to the various signal routes that are activated in unfolded protein response (UPR). The Grp78, Grp94, CHOP, MTJ1 and HMOX1 genes expressions demonstrate UPR activity. In this study, we investigated the UPR gene expressions in larynx epidermoid carcinoma (HEp2) to which dexamethasone (dex) was applied. HEp2 cells were administered for 48 h with different combinations using 0.1 μM and 1 μM dex, 1 mM phenyl butyric acid (PBA) and 100 ng/ml lipopolysaccharide (LPS). The Grp78, Grp94, CHOP, MTJ1 and HMOX1 genes expression was determined using quantitative RT-PCR. The Grp78, MTJ1 and HMOX1 gene expression increased with the administration of 1 µM dex. CHOP expression, on the other hand, decreased with 0.1 µM dex. When dex was combined with LPS, nearly all gene expressions decreased. The increase in Grp78, Grp94, HMOX1 and MTJ1 gene expression was greater in groups in which dex was administered in combination with PBA than in groups in which dex was administered alone. Dex in low dose (0.1 μM) caused a decrease in CHOP expression in HEp2 cells and an increase in Grp78 expression, in particular. The changes in UPR genes expressions may lead to the extended survival of the cells. |
| Anahtar Kelimeler |
| CHOP | Dexamethasone | Endoplasmic reticulum stress | Grp78 | HEp2 | Lipopolysaccharide | Phenyl butyric acid |
