Ouabain Targets the Unfolded Protein Response for Selective Killing of HepG2 Cells During Glucose Deprivation
Yazarlar (8)
Tülay Özdemir Gazi State Hospital, Samsun, Türkiye
Rukiye Nar
Pamukkale Üniversitesi, Türkiye
Pamukkale Üniversitesi, Türkiye
Veli Kılınç
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
Hasan Alaçam
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
Osman Saliş
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
Aynur Düzgün
Tirebolu State Hospital, Türkiye
Tirebolu State Hospital, Türkiye
Doç. Dr. Sedat GÜLTEN Kastamonu Üniversitesi, Türkiye
Abdülkerim Bedir
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Cancer Biotherapy and Radiopharmaceuticals | ||
| Dergi ISSN | 1084-9785 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 10-2012 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-10-2012 |
| Cilt / Sayı / Sayfa | 27 / 8 / 457–463 | DOI | 10.1089/cbr.2011.1138 |
| Makale Linki | http://dx.doi.org/10.1089/cbr.2011.1138 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| Ouabain is a cardiotonic steroid and specific inhibitor of the Na+/K+-ATPase. The relationship between ouabain treatment and the unfolded protein response (UPR) in cells is not precisely understood. Therefore, we studied the possible effects of ouabain on proliferation, apoptosis, and the UPR. HepG2 cells were cultured overnight and then treated with various concentrations of ouabain (0.75 to 750 nM) in the absence or presence of 10 mM 2-deoxyglucose (2-DG) for 48 hours. We also used real-time polymerase chain reaction to obtain quantitative measurements of expression levels of Grp78, Grp94, CHOP, MTJ-1, HKII, MDR-1, MRP-1, HO-1, and Par-4. Cell number, viability, and proliferation of HepG2 cells were monitored with a real-time cell analyzer system (xCELLigence). We show that ouabain modulates the UPR transcription program and induces cell death in glucose-deprived tumor cells. Ouabain at all … |
| Anahtar Kelimeler |
| 2-deoxyglucose | CHOP | Grp78 | HepG2 | ouabain | unfolded protein response |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 14 |
| Scopus | 11 |
| Google Scholar | 26 |