| Yazarlar |
Rukiye Nar
Pamukkale Üniversitesi, Türkiye |
|
Abdülkerim Bedir
|
|
Hasan Alaçam
Türkiye |
|
Veli Kılınç
Türkiye |
|
Bahattin Avcı
Ondokuz Mayıs Üniversitesi, Türkiye |
|
Osman Saliş
|
Doç. Dr. Sedat GÜLTEN
Kastamonu Üniversitesi, Türkiye |
| Özet |
| Our purpose in this study is to analyze mitochondrial DNA (mtDNA) lesion frequencies and mtDNA(4977) deletion in HepG2 cells to examine the effects of ouabain on mtDNA. HepG2 cells were treated with 0.75, 7.5, 75, and 750 nM of ouabain for 24 h in the presence and absence of 10 mM 2-deoxyglucose (2-DG). The frequency of mtDNA(4977) deletions and mitochondrial lesions were determined by real-time polymerase chain reaction. A ≥ 1.2-fold change or greater was considered significant. Ouabain doses of 750, 75, and 7.5 nM alone increased the frequency of mtDNA(4977) deletions 1.39, 1.92, and 1.44 times, respectively. The 750 and 75 nM ouabain doses combined with 2-DG increased the mtDNA(4977) deletion frequency 4.94 and 1.57 times, respectively. The 750 and 75 nM ouabain doses alone increased the mtDNA lesion frequency 2.5 and 1.5 times, respectively. The 750 nM ouabain dose combined with 2-DG increased the mtDNA lesion frequency 2.28 times. The 7.5 nM ouabain dose alone and combined with 2-DG decreased the mtDNA lesion frequency 0.67 and 0.45 times, respectively. Ouabain alone and when combined with 2-DG increases mtDNA lesion and mtDNA(4977) deletion frequencies. This supports the thesis that ouabain creates oxidative stress and induces DNA damage and apoptosis. |
| Anahtar Kelimeler |
| Ouabain | Common deletion | 2-Deoxyglucose | Mitochondrial lesion frequency | RT-PCR |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | TUMOR BIOLOGY |
| Dergi ISSN | 1010-4283 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2012 |
| Cilt No | 33 |
| Sayı | 6 |
| Sayfalar | 2107 / 2115 |
| Doi Numarası | 10.1007/s13277-012-0470-8 |
| Makale Linki | http://dx.doi.org/10.1007/s13277-012-0470-8 |
| Atıf Sayıları | |
| WoS | 2 |
| SCOPUS | 3 |
| Google Scholar | 6 |
