The effect of ouabain on mitochondrial DNA damage in HepG2 cell lines
Yazarlar (7)
Rukiye Nar
Pamukkale Üniversitesi, Türkiye
Pamukkale Üniversitesi, Türkiye
Abdülkerim Bedir
Hasan Alaçam
Türkiye
Türkiye
Veli Kılınç
Türkiye
Türkiye
Prof. Dr. Bahattin Avcı Ondokuz Mayıs Üniversitesi, Türkiye
Osman Saliş
Doç. Dr. Sedat GÜLTEN Kastamonu Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Tumour Biology the Journal of the International Society for Oncodevelopmental Biology and Medicine | ||
| Dergi ISSN | 1423-0380 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2012 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 15-08-2012 |
| Cilt / Sayı / Sayfa | 33 / 6 / 2107–2115 | DOI | 10.1007/s13277-012-0470-8 |
| Makale Linki | http://dx.doi.org/10.1007/s13277-012-0470-8 | ||
| UAK Araştırma Alanları |
Tıbbi Biyokimya
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| Özet |
| Our purpose in this study is to analyze mitochondrial DNA (mtDNA) lesion frequencies and mtDNA4977 deletion in HepG2 cells to examine the effects of ouabain on mtDNA. HepG2 cells were treated with 0.75, 7.5, 75, and 750 nM of ouabain for 24 h in the presence and absence of 10 mM 2-deoxyglucose (2-DG). The frequency of mtDNA4977 deletions and mitochondrial lesions were determined by real-time polymerase chain reaction. A ≥1.2-fold change or greater was considered significant. Ouabain doses of 750, 75, and 7.5 nM alone increased the frequency of mtDNA4977 deletions 1.39, 1.92, and 1.44 times, respectively. The 750 and 75 nM ouabain doses combined with 2-DG increased the mtDNA4977 deletion frequency 4.94 and 1.57 times, respectively. The 750 and 75 nM ouabain doses alone increased the mtDNA lesion frequency 2.5 and 1.5 times, respectively. The 750 nM ouabain dose combined … |
| Anahtar Kelimeler |
| Ouabain | Common deletion | 2-Deoxyglucose | Mitochondrial lesion frequency | RT-PCR |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 2 |
| Scopus | 3 |
| Google Scholar | 6 |