| Yazarlar |
Doç. Dr. Adem Yavuz SÖNMEZ
Kastamonu Üniversitesi, Türkiye |
Arş. Gör. Dr. Rahmi Can ÖZDEMİR
Kastamonu Üniversitesi, Türkiye |
Doç. Dr. Soner BİLEN
Kastamonu Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Keriman YÜRÜTEN ÖZDEMİR
Kastamonu Üniversitesi, Türkiye |
| Özet |
| This study investigated the effect of cyclopamine, a hedgehog signaling inhibitor, on immune-related gene expression in rainbow trout head kidney leukocytes in vitro. At 1h sampling time COX 2, IFN-2, TNF-α, C3, MHC-II, Il-1β, IL-12, IL-10, and IL-6 increased significantly in groups exposed to cyclopamine at 30 μg/ml. However, in groups exposed to 10 and 20 μg/ml cyclopamine, the expression of these genes decreased significantly. At 4h sampling time, levels of COX-2, IFN-1, IFN-2, TNF-α, TGF-β, IgT, and MHC-II in groups exposed to 20 μg/ml cyclopamine increased. At 8h sampling time, COX2, IFN-Reg, and TNF-α expression in groups exposed to 10 and 30 μg/ml cyclopamine increased significantly compared to those in the controls. Conversely the expression decreased significantly for almost all other genes. At 12h sampling time, almost all genes increased significantly in groups exposed to 10 μg/ml cyclopamine compared to those in the controls. Notably, after 24h, the gene expression in all groups significantly decreased compared to the controls. Our results suggest that the activation of cyclopamine can be a useful tool for the examination of immune-related gene expression in the rainbow trout. |
| Anahtar Kelimeler |
| cyclopamine | rainbow trout | leukocyte | immune responses | gene expression |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH |
| Dergi ISSN | 0792-156X |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2018 |
| Cilt No | 70 |
| Sayfalar | 1 / 8 |
| Makale Linki | http://evols.library.manoa.hawaii.edu/bitstream/10524/59402/70.2018.1560.Ozdemir.pdf |
