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Cyclopamine Induced Expression of Immune-related Genes in Rainbow trout (Oncorhynchus mykiss) Head Kidney Leukocytes   
Yazarlar
Adem Yavuz Sönmez
Kastamonu Üniversitesi, Türkiye
Arş. Gör. Rahmi Can ÖZDEMİR
Kastamonu Üniversitesi, Türkiye
Doç. Dr. Soner BİLEN
Kastamonu Üniversitesi, Türkiye
Dr. Öğr. Üyesi Keriman YÜRÜTEN ÖZDEMİR
Kastamonu Üniversitesi, Türkiye
Özet
This study investigated the effect of cyclopamine, a hedgehog signaling inhibitor, on immune-related gene expression in rainbow trout head kidney leukocytes in vitro. At 1h sampling time COX 2, IFN-2, TNF-α, C3, MHC-II, Il-1β, IL-12, IL-10, and IL-6 increased significantly in groups exposed to cyclopamine at 30 μg/ml. However, in groups exposed to 10 and 20 μg/ml cyclopamine, the expression of these genes decreased significantly. At 4h sampling time, levels of COX-2, IFN-1, IFN-2, TNF-α, TGF-β, IgT, and MHC-II in groups exposed to 20 μg/ml cyclopamine increased. At 8h sampling time, COX2, IFN-Reg, and TNF-α expression in groups exposed to 10 and 30 μg/ml cyclopamine increased significantly compared to those in the controls. Conversely the expression decreased significantly for almost all other genes. At 12h sampling time, almost all genes increased significantly in groups exposed to 10 μg/ml cyclopamine compared to those in the controls. Notably, after 24h, the gene expression in all groups significantly decreased compared to the controls. Our results suggest that the activation of cyclopamine can be a useful tool for the examination of immune-related gene expression in the rainbow trout.
Anahtar Kelimeler
Cyclopamine | Gene expression | Immune responses | Leukocyte | Rainbow trout
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı The Israeli Journal of Aquaculture
Dergi ISSN 0792-156X
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce
Basım Tarihi 12-2018
Cilt No 702018
Sayfalar 1 / 8
Makale Linki http://evols.library.manoa.hawaii.edu/bitstream/10524/59402/70.2018.1560.Ozdemir.pdf
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
SCOPUS 8

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