Comparison of direct and sandwich type immunoassays on electrospun nanofibers using of metal organic frameworks as a fluorescence probe

Kırbay, Fatma Öztürk; YAZGAN, İdris; Demirkol, Dilek Odaci

doi:10.1016/j.snb.2022.132621

Comparison of direct and sandwich type immunoassays on electrospun nanofibers using of metal organic frameworks as a fluorescence probe

Yazarlar (3)

Fatma Öztürk Kırbay
Ege Üniversitesi, Türkiye

Prof. Dr. İdris YAZGAN Kastamonu Üniversitesi, Türkiye

Dilek Odaci Demirkol Ege Üniversitesi, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Sensors and Actuators B Chemical (Q1)
Dergi ISSN	0925-4005 Dergi Bilgileri (2022)
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	İngilizce	Basım Tarihi	12-2022
Kabul Tarihi	–	Yayınlanma Tarihi	01-12-2022
Cilt / Sayı / Sayfa	372 / 1 / 132621–0	DOI	10.1016/j.snb.2022.132621
Makale Linki	http://dx.doi.org/10.1016/j.snb.2022.132621
UAK Araştırma Alanları	Biyokimya

Özet

Here, colloidal fluorescent Fe (III) metal organic frameworks (FeMOFs) were synthesized using FeCl3 as Fe³⁺ ion source and lactose 1-aminonaphthalene (L1AN) as an organic linker by one-step synthesis approach. C-reactive protein antibody (Anti-CRP) was conjugated to L1AN-FeMOF through amide bond formation, which was then applied to the detection of C-reactive protein (CRP) using sandwich-type and immunometric assays. To cover 96-well plate surfaces, polycaprolactone and poly (amic acid) (PCL/PAA) electrospun nanofibers were synthesized. Limit of detection (LOD) for CRP was obtained as 4.8 pg/mL for the sandwich assay and 9.0 pg/mL for the immunometric assay in buffer systems using high throughput sensing approach. Real sample applications in artificial saliva and simulated serum validated the reliability of the approach. Selectivity control tests were performed using common serum-based interferents, for which minimum to no interference was observed. Based on the findings, the developed assay can be offered as a new approach in the detection of CRP in clinical samples.

Anahtar Kelimeler

Science Direct

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
Scopus	20

Comparison of direct and sandwich type immunoassays on electrospun nanofibers using of metal organic frameworks as a fluorescence probe

Dergi Adı	SENSORS AND ACTUATORS B-CHEMICAL
Kısa Adı	SENSOR ACTUAT B-CHEM
Yayıncı	ELSEVIER SCIENCE SA
Açık Erişim	Hayır
E-ISSN	0925-4005
Wos Quartile	Q1
Tarandığı Indeksler	SCIE
WoS Kategoriler	CHEMISTRY, ANALYTICAL \| ELECTROCHEMISTRY \| INSTRUMENTS & INSTRUMENTATION
Scopus Kategoriler

Comparison of direct and sandwich type immunoassays on electrospun nanofibers using of metal organic frameworks as a fluorescence probe

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