Use of Pyranose Oxidase Enzyme in Inhibitor Biosensing
Yazarlar (4)
Prof. Dr. Tuba Aydin Ege Üniversitesi, Türkiye
Prof. Dr. Dilek Odaci Ege Üniversitesi, Türkiye
Prof. Dr. İdris YAZGAN Kastamonu Üniversitesi, Türkiye
Prof. Dr. Suna Timur Ege Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Analytical Letters
Dergi ISSN 0003-2719 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce Basım Tarihi 08-2008
Kabul Tarihi Yayınlanma Tarihi 24-07-2008
Cilt / Sayı / Sayfa 41 / 11 / 2088–2096 DOI 10.1080/00032710802209276
Makale Linki http://www.tandfonline.com/doi/abs/10.1080/00032710802209276
Özet
An inhibition based biosensing system was developed for the determination of glutathione (GSH) and ethanol (EtOH) as pyranose oxidase (PyOx) inhibitors. The PyOx was immobilized in carbon paste electrode and the amperometric detection of hydrogen peroxide through the enzymatic reaction was monitored atμ+μ0.9μV versus Ag/AgCl. In addition to the optimization studies, analytical characteristics and the effect of various compounds on the biosensor response were researched. Finally, the proposed system was applied to analyze GSH and EtOH in real matrices. Copyright © Taylor & Francis Group, LLC.
Anahtar Kelimeler
Ethanol | Glutathione (GSH) | Inhibitor based biosensor | Pyranose oxidase (PyOx)