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Use of Pyranose Oxidase Enzyme in Inhibitor Biosensing   
Yazarlar
Tuba Aydin
Dilek Odaci
Doç. Dr. İdris YAZGAN
Kastamonu Üniversitesi, Türkiye
Suna Timur
Ege Üniversitesi, Türkiye
Özet
An inhibition based biosensing system was developed for the determination of glutathione (GSH) and ethanol (EtOH) as pyranose oxidase (PyOx) inhibitors. The PyOx was immobilized in carbon paste electrode and the amperometric detection of hydrogen peroxide through the enzymatic reaction was monitored atμ+μ0.9μV versus Ag/AgCl. In addition to the optimization studies, analytical characteristics and the effect of various compounds on the biosensor response were researched. Finally, the proposed system was applied to analyze GSH and EtOH in real matrices. Copyright © Taylor & Francis Group, LLC.
Anahtar Kelimeler
Ethanol | Glutathione (GSH) | Inhibitor based biosensor | Pyranose oxidase (PyOx)
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Analytical Letters
Dergi ISSN 0003-2719
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce
Basım Tarihi 08-2008
Cilt No 41
Sayı 11
Sayfalar 2088 / 2096
Doi Numarası 10.1080/00032710802209276
Makale Linki http://www.tandfonline.com/doi/abs/10.1080/00032710802209276