Quantification of Antioxidant Ability Against Lipid Peroxidation with an ‘Area Under Curve’ Approach

BAKIR, Temel Kan; Sönmezoglu, İnci; Apak, Reşat

doi:10.1007/s11746-016-2918-2

Quantification of Antioxidant Ability Against Lipid Peroxidation with an ‘Area Under Curve’ Approach

Yazarlar (3)

Prof. Dr. Temel Kan BAKIR Kastamonu Üniversitesi, Türkiye

İnci Sönmezoglu
Yıldız Teknik Üniversitesi, Türkiye

Reşat Apak
Istanbul Üniversitesi, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	JAOCS Journal of the American Oil Chemists Society
Dergi ISSN	0003-021X Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI
Makale Dili	İngilizce	Basım Tarihi	01-2017
Kabul Tarihi	–	Yayınlanma Tarihi	18-11-2016
Cilt / Sayı / Sayfa	94 / 1 / 77–88	DOI	10.1007/s11746-016-2918-2
Makale Linki	http://doi.wiley.com/10.1007/s11746-016-2918-2
UAK Araştırma Alanları	Fiziksel Kimya

Özet

When oxygen is passed through a linoleic acid (LA) emulsion containing copper(II), primary (hydroperoxides) and secondary oxidation products (aldehydes and ketones) are formed, monitored by ferric thiocyanate (Fe(III)-SCN) and thiobarbituric acid reactive substances (TBARS) colorimetry, respectively. As total antioxidant capacity (TAC) against lipid peroxidation was not quantified before, both methods were adapted to an ‘area under curve (AUC)’ approach. LA peroxidation followed pseudo-first order kinetics in aerated emulsions. Absorbance changes as a function of incubation time exhibited sigmoidal curves, enabling the calculation of ‘area under curve’ (AUC) and net AUC = AUCblank−AUCsample, standard calibration curve as net AUC versus concentration, and trolox-equivalent antioxidant capacity of the tested compounds. Garlic extract showed an antioxidative effect on hydroperoxide formation, but a prooxidative effect on TBARS. Although inhibition of lipid peroxidation was described qualitatively before, it was not evaluated quantitatively, e.g., the trolox-equivalent antioxidant capacities (TEAC values) of antioxidants with respect to their inhibitive effect against lipid peroxidation were not calculated and compared. Additionally, real-time monitoring of lipid oxidation products requires highly sophisticated but costly instrumental techniques, but no single oxidation product is a direct measure of lipid oxidation or its antioxidative prevention. The AUC approach is the first quantitative method measuring antioxidant protection against lipid oxidation, with a slightly different order of antioxidative effectiveness from reductive assays because of interfacial effects.

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Atıf Sayıları
Web of Science	7
Scopus	7

Quantification of Antioxidant Ability Against Lipid Peroxidation with an ‘Area Under Curve’ Approach

Dergi Adı	JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Yayıncı	John Wiley & Sons Inc.
Açık Erişim	Hayır
ISSN	0003-021X
E-ISSN	1558-9331
CiteScore	4,3
SJR	0,468
SNIP	0,717

Quantification of Antioxidant Ability Against Lipid Peroxidation with an ‘Area Under Curve’ Approach

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