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Comparative bioinformatics analysis and abiotic stress responses of expansin proteins in Cucurbitaceae members: watermelon and melon     
Yazarlar
Çınar Yiğit İncili
Büşra Arslan
Doç. Dr. Esra Nurten YER ÇELİK Doç. Dr. Esra Nurten YER ÇELİK
Türkiye
Dr. Öğr. Üyesi Ferhat ULU Dr. Öğr. Üyesi Ferhat ULU
Türkiye
Erdoğan Horuz
Prof. Dr. Mehmet Cengiz BALOĞLU Prof. Dr. Mehmet Cengiz BALOĞLU
Türkiye
Ebrar Çağlıyan
Gamze Burcu
Dr. Öğr. Üyesi Aslı UĞURLU BAYARSLAN Dr. Öğr. Üyesi Aslı UĞURLU BAYARSLAN
Türkiye
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU Prof. Dr. Yasemin ÇELİK ALTUNOĞLU
Türkiye
Özet
Watermelon and melon are members of the Cucurbitaceae family including economically significant crops in the world. The expansin protein family, which is one of the members of the cell wall, breaks down the non-covalent bonds between cell wall polysaccharides, causing pressure-dependent cell expansion. Comparative bioinformatics and molecular characterization analysis of the expansin protein family were carried out in the watermelon (Citrullus lanatus) and melon (Cucumis melo) plants in the study. Gene expression levels of expansin family members were analyzed in leaf and root tissues of watermelon and melon under ABA, drought, heat, cold, and salt stress conditions by quantitative real-time PCR analysis. After comprehensive searches, 40 expansin proteins (22 ClaEXPA, 14 ClaEXPLA, and 4 ClaEXPB) in watermelon and 43 expansin proteins (19 CmEXPA, 15 CmEXPLA, 3 CmEXPB, and 6 CmEXPLB) in melon were identified. The greatest orthologous genes were identified with soybean expansin genes for watermelon and melon. However, the latest divergence time between orthologous genes was determined with poplar expansin genes for watermelon and melon expansin genes. ClaEXPA-04, ClaEXPA-09, ClaEXPB-01, ClaEXPB-03, and ClaEXPLA-13 genes in watermelon and CmEXPA-12, CmEXPA-10, and CmEXPLA-01 genes in melon can be involved in tissue development and abiotic stress response of the plant. The current study combining bioinformatics and experimental analysis can provide a detailed characterization of the expansin superfamily which has roles in growth and reaction to the stress of the plant. The study ensures detailed data for future studies examining gene functions including the roles in plant growth and stress conditions.
Anahtar Kelimeler
Abiotic stresses | Characterization | Citrullus lanatus | Cucumis melo | Expansin genes
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Protoplasma
Dergi ISSN 0033-183X
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q2
Makale Dili Türkçe
Basım Tarihi 07-2023
Cilt No 260
Doi Numarası 10.1007/s00709-022-01793-8
Makale Linki http://dx.doi.org/10.1007/s00709-022-01793-8