| Yazarlar |
Mehmet Burak Ateş
Selçuk Üniversitesi, Türkiye |
|
Mustafa Ortatatlı
Selçuk Üniversitesi, Türkiye |
|
Halis Uğuz
Türkiye |
|
Özgür Özdemir
Selçuk Üniversitesi, Türkiye |
Doç. Dr. Funda TERZİ
Kastamonu Üniversitesi, Türkiye |
|
Mustafa Kemal Çiftçi
İstanbul Gelişim Üniversitesi, Türkiye |
|
Fatih Hatipoğlu
Selçuk Üniversitesi, Türkiye |
| Özet |
| 1. Aflatoxins (AFs) are metabolites which especially have toxic effects on proteins, and are detoxified by the aflatoxin-B1 aldehyde reductase (AFAR) pathway. In this pathway, the aldo-keto reductase family 7, member A2 (AKR7A2) enzyme, which is controlled by nucleic-related erythroid factor 2 (Nrf2), plays an active role. However, data on the efficacy of this critical pathway in broilers is limited. 2. The aim of the following study was to investigate the changes in the expression levels of AKR7A2, Nrf2, and caspase-3, and the effects of Nigella sativa seeds (NS), thymoquinone (TMQ), and bentonite (BNT) in broilers exposed to AFs. 3. One-hundred broilers were divided into ten groups (control (CNT); AF; NS; TMQ; BNT; AF+TMQ; AF+NS; AF+BNT; AF+BNT+NS; AF+BNT+TMQ) and fed for 28 d. AF, TMQ, NS and BNT were added to diets at levels of 2 mg/kg, 300 mg/kg, 50 g/kg and 10 g/kg respectively. 4. The addition of AF to the diet decreased AKR7A2 and Nrf2 levels dramatically, but increased caspase-3 (P < 0.01). TMQ, NS and BNT additions to the diet eliminated all negative effects caused by AF (P < 0.01); and AKR7A2 and Nrf2 were further raised in TMQ and NS groups when compared to the control group. TMQ and NS showed a positive effect on detoxification parameters when given together with BNT. 5. Supplementation with NS and TMQ enhanced AF detoxification via the AFAR pathway, by increasing AKR7A2 and Nrf2 levels, in addition to reducing hepatocyte apoptosis. |
| Anahtar Kelimeler |
| AKR7A2 | apoptosis | black cumin | liver | Mycotoxin | Nrf2 |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | British Poultry Science |
| Dergi ISSN | 0007-1668 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2022 |
| Cilt No | 63 |
| Sayı | 3 |
| Sayfalar | 332 / 339 |
| Doi Numarası | 10.1080/00071668.2021.1998366 |
| Makale Linki | https://www.tandfonline.com/doi/full/10.1080/00071668.2021.1998366 |
| Atıf Sayıları | |
| SCOPUS | 13 |
