Morphology of Sodium Salt of Calf Thymus DNA on Mica Alumina and Silica Surfaces: Effect of Solvent and Drying Method
Yazarlar (2)
Doç. Dr. Senem YETGİN Kastamonu Üniversitesi, Türkiye
Devrim Balköse Izmir Yüksek Teknoloji Enstitüsü, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Drying Technology (Q2)
Dergi ISSN 0737-3937 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce Basım Tarihi 04-2017
Cilt / Sayı / Sayfa 35 / 8 / 1007–1019 DOI 10.1080/07373937.2016.1227833
Makale Linki https://www.tandfonline.com/doi/full/10.1080/07373937.2016.1227833
Özet
Investigation of morphology of deoxyribonucleotide triphosphate (DNA) dried on different surfaces by atomic force microscopy (AFM) is important in DNA research that is focused on subjects of condensation for gene therapy, sizing, DNA mapping, and cancer examination. The solvent, the surface type, and the method of drying effect the morphology of DNA on solid surfaces. Ethanol and water were used as solvents, flat mica, silica, and alumina surfaces were used as the substrates in the present study. Different methods such as ambient air-drying, N2-forced flow regime drying, and freeze-drying have been applied to droplets of DNA solutions in water or ethanol on the substrates. Forced flow drying regime causes nonlinear DNA attachment on the surface and self-assembly. DNA vertical distance on mica surface was found to be 6 and 1.4 nm for DNA dried in ambient air from ethanol and water solutions …
Anahtar Kelimeler
AFM | alumina | DNA | drying | mica | silica | solvent