Calf thymus DNA characterization and its adsorption on different silica surfaces
Yazarlar (2)
Doç. Dr. Senem YETGİN Kastamonu Üniversitesi, Türkiye
Devrim Balkose Izmir Yüksek Teknoloji Enstitüsü, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
|
||
| Dergi Adı | Rsc Advances (Q2) | ||
| Dergi ISSN | 2046-2069 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2015 |
| Cilt / Sayı / Sayfa | 5 / 71 / 57950–57959 | DOI | 10.1039/c5ra01810b |
| Makale Linki | http://xlink.rsc.org/?DOI=C5RA01810B | ||
| Özet |
| DNA adsorption is the initial stage of gene therapy for drug delivery systems and solid phase extraction methods of DNA purification. High pore volume and high adsorption capacity are simple requirements not only for producing ‘smart’ drug delivery systems but also the development of purification kits. Silica is the most used material for this purpose. The present study aimed at elucidating the calf thymus DNA biosorption process by the characterization of calf thymus DNA and silica to increase the efficiency of the currently used silica material. Mesoporous silica has long been used for DNA adsorption and silica aerogel is the new adsorbent investigated in the present study. When DNA solution was freeze dried on a silica wafer, self-assembled super helices formed as shown by atomic microscopy (AFM). Thus DNA existed not as single molecules but as large sized agglomerates in water. Thus it could be adsorbed … |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 12 |
| Google Scholar | 19 |