| Yazarlar |
Ümit Muhammet Koçyiğit
Türkiye |
|
Murat Doğan
Türkiye |
Doç. Dr. Halit MUĞLU
Türkiye |
|
Parham Taslımı
Türkiye |
|
Burak Tüzün
Sivas Cumhuriyet Üniversitesi, Türkiye |
|
Hasan Yakan
Türkiye |
|
Halil Bal
Türkiye |
|
Emre Güzel
Türkiye |
|
İlhami Gülçin
Türkiye |
| Özet |
| Design, synthesis, structural elucidation, and investigation of cytotoxic and antimicrobial activity, butyrylcholinesterase (BChE), and acetylcholinesterase (AChE) enzyme inhibition effects of isatin-thiosemicarbazone hybrid compounds (1–15) are reported in this study. Hybrid compounds (14 and 15) were synthesized, isolated, and characterized for the first time. FT-IR, 1H NMR, and 13C NMR spectroscopic methods and elemental analysis were used to characterize the structures of the compounds. In the enzymatic evaluation, hybrid compound 13 was observed as the most potent inhibitor of AChE with a Kİ value of 0.94 ± 0.13 µM (all compound Kİ values between 0.94 ± 0.13 and 4.47 ± 0.92), also this compound was observed as the most potent inhibitor of BChE with a Kİ value of 0.82 ± 0.11 µM (all compounds had Kİ values between of 0.82 ± 0.11 and 3.48 ± 0.92). Almost all compounds were shown better inhibition profile than standard compound. In the theoretical calculations, the comparison of the biological activities of isatin-thiosemicarbazone hybrid derivatives against enzymes was studied. The enzymes studied in docking calculations are AChE and BChE. Then, ADME/T analysis was conducted to examine the drug properties of these derivatives. Besides, the antimicrobial activity of these molecules was investigated by the microdilution method according to Clinical Laboratory Standards Institute (CLSI) criteria in the study. Cytotoxic activity of isatin-thiosemicarbazone hybrids was determined by the XTT cell viability assay on human breast cancer cell lines MCF-7 and MDA-MB-231. Among the hybrid compounds, compound 8 exhibited the most potent cytotoxic activity with IC50 values of 23.42 ± 0.21 µg/mL and 19.68 ± 0.23 µg/mL on MCF-7 and MDA-MB-231 cell lines, respectively. Overall, the hybridization of isatin and thiosemicarbazone skeleton has played an essential role in the inhibition of enzymes and cytotoxic activity. |
| Anahtar Kelimeler |
| 5-methoxyisatin | Antimicrobial activity | Cytotoxic activity | Enzyme inhibition activity | Molecular docking | Thiosemicarbazone |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Journal of Molecular Structure |
| Dergi ISSN | 0022-2860 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2022 |
| Cilt No | 1264 |
| Sayı | 1 |
| Sayfalar | 133249 / 0 |
| Doi Numarası | 10.1016/j.molstruc.2022.133249 |
| Makale Linki | http://dx.doi.org/10.1016/j.molstruc.2022.133249 |
| Atıf Sayıları | |
| SCOPUS | 26 |
