Properties of immobilized glucose oxidase and enhancement of enzyme activity

Ateş, Selma; İÇLİ, Nesrin

doi:10.3109/10731199.2012.731415

Properties of immobilized glucose oxidase and enhancement of enzyme activity

Yazarlar (2)

Selma Ateş Gazi Üniversitesi, Türkiye

Doç. Dr. Nesrin İÇLİ Undersecretariat Of Customs, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Artificial Cells Nanomedicine and Biotechnology (Q2)
Dergi ISSN	2169-1401 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	İngilizce	Basım Tarihi	07-2013
Cilt / Sayı / Sayfa	41 / 4 / 264–268	DOI	10.3109/10731199.2012.731415
Makale Linki	http://www.tandfonline.com/doi/full/10.3109/10731199.2012.731415

Özet

In this study; glucose oxidase (GOD) and MnO2 were immobilized in alginate gel beads using the entrapment method and effects of oxygen vectors on immobilized GOD activity were investigated. The specific activity of free enzyme was 1.3 units/mg protein whereas that of immobilized enzyme was 8.4 × 10^-1 units/mg protein. The Km values for free and immobilized enzyme were found to be 2.7 mM, 2.9 mM; Vmax values 3.64 × 10 ^-1 μmol/min.mL, 2.61 × 10^-1 μmol/min.mL, respectively. When 4% (v/v) silicone oil or soybean oil was used, the activity of immobilized GOD increased 1.7 times and 1.3 times, respectively, compared to media without these oils. © 2013 Informa Healthcare USA, Inc.

Anahtar Kelimeler

Calcium alginate gel | Gluconic acid | Immobilized glucose oxidase | Silicone oil | Soybean oil

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
Web of Science	13
Scopus	15

Properties of immobilized glucose oxidase and enhancement of enzyme activity

Dergi Adı	Artificial Cells Nanomedicine and Biotechnology
Yayıncı	Taylor and Francis Ltd.
Açık Erişim	Evet
ISSN	2169-1401
E-ISSN	2169-141X
CiteScore	8,8
SJR	0,739
SNIP	0,954

Properties of immobilized glucose oxidase and enhancement of enzyme activity

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