| Yazarlar |
Selma Ateş
|
Doç. Dr. Nesrin İÇLİ
Türkiye |
| Özet |
| In this study; glucose oxidase (GOD) and MnO2 were immobilized in alginate gel beads using the entrapment method and effects of oxygen vectors on immobilized GOD activity were investigated. The specific activity of free enzyme was 1.3 units/mg protein whereas that of immobilized enzyme was 8.4 × 10-1 units/mg protein. The Km values for free and immobilized enzyme were found to be 2.7 mM, 2.9 mM; Vmax values 3.64 × 10 -1 μmol/min.mL, 2.61 × 10-1 μmol/min.mL, respectively. When 4% (v/v) silicone oil or soybean oil was used, the activity of immobilized GOD increased 1.7 times and 1.3 times, respectively, compared to media without these oils. © 2013 Informa Healthcare USA, Inc. |
| Anahtar Kelimeler |
| Calcium alginate gel | Gluconic acid | Immobilized glucose oxidase | Silicone oil | Soybean oil |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Artificial Cells, Nanomedicine, and Biotechnology |
| Dergi ISSN | 2169-1401 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2013 |
| Cilt No | 41 |
| Sayı | 4 |
| Sayfalar | 264 / 268 |
| Doi Numarası | 10.3109/10731199.2012.731415 |
| Makale Linki | http://www.tandfonline.com/doi/full/10.3109/10731199.2012.731415 |
| Atıf Sayıları | |
| WoS | 10 |
| SCOPUS | 10 |
