Properties of immobilized glucose oxidase and enhancement of enzyme activity
 
Yazarlar (2)
Prof. Dr. Selma Ateş Gazi Üniversitesi, Türkiye
Doç. Dr. Nesrin İÇLİ Undersecretariat Of Customs, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Artificial Cells Nanomedicine and Biotechnology
Dergi ISSN 2169-1401 Dergi Bilgileri (2013)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 07-2013
Kabul Tarihi – Yayınlanma Tarihi 30-10-2012
Cilt / Sayı / Sayfa 41 / 4 / 264–268 DOI 10.3109/10731199.2012.731415
Makale Linki http://www.tandfonline.com/doi/full/10.3109/10731199.2012.731415
UAK Araştırma Alanları
Toksikoloji
Özet
In this study; glucose oxidase (GOD) and MnO2 were immobilized in alginate gel beads using the entrapment method and effects of oxygen vectors on immobilized GOD activity were investigated. The specific activity of free enzyme was 1.3 units/mg protein whereas that of immobilized enzyme was 8.4 × 10-1 units/mg protein. The Km values for free and immobilized enzyme were found to be 2.7 mM, 2.9 mM; Vmax values 3.64 × 10 -1 μmol/min.mL, 2.61 × 10-1 μmol/min.mL, respectively. When 4% (v/v) silicone oil or soybean oil was used, the activity of immobilized GOD increased 1.7 times and 1.3 times, respectively, compared to media without these oils. © 2013 Informa Healthcare USA, Inc.
Anahtar Kelimeler
Calcium alginate gel | Gluconic acid | Immobilized glucose oxidase | Silicone oil | Soybean oil
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Web of Science 13
Scopus 15
Properties of immobilized glucose oxidase and enhancement of enzyme activity

Paylaş

İletişim
  • Kastamonu Üniversitesi Rektörlüğü, Kuzeykent Kampüsü, Merkez/Kastamonu
  • unis@kastamonu.edu.tr 03662801000
Sosyal Media
Talentsoft Yazılım Talent Yazılım
Tasarım ve Programlama #2025