| Yazarlar |
Ferdane Danışman Kalındemirtaş
Türkiye |
Doç. Dr. Dilşad ÖZERKAN
Kastamonu Üniversitesi, Türkiye |
|
İshak Afşin Kariper
Türkiye |
|
Huri Bulut
Türkiye |
| Özet |
| Recently, nanocarriers have been made to eliminate the disadvantages of chemotherapeutic agents by nanocarriers. Nanocarriers show their efficacy through their targeted and controlled release. In this study, 5-fluorouracil (5FU) was loaded into ruthenium (Ru)-based nanocarrier (5FU-RuNPs) for the first time to eliminate the disadvantages of 5FU, and its cytotoxic and apoptotic effects on HCT116 colorectal cancer cells were compared with free 5FU. 5FU-RuNPs with a size of approximately 100 nm showed a 2.61-fold higher cytotoxic effect compared to free 5FU. Apoptotic cells were detected by Hoechst/propidium iodide double staining, and the expression levels of BAX/Bcl-2 and p53 proteins, in which apoptosis occurred intrinsically, were revealed. In addition, 5FU-RuNPs was also found to reduce multidrug resistance (MDR) according to BCRP/ABCG2 gene expression levels. When all the results were evaluated, the fact that Ru-based nanocarriers alone did not cause cytotoxicity proved that they were ideal nanocarriers. Moreover, 5FU-RuNPs did not show any significant effect on the cell viability of normal human epithelial cell lines BEAS-2B. Consequently, the 5FU-RuNPs synthesized for the first time may be ideal candidates for cancer treatment because they can minimize the potential drawbacks of free 5FU. |
| Anahtar Kelimeler |
| Ruthenium-based nanoparticles,5FU,Colorectal cancer,Apoptosis,Cytotoxicity |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Journal of Flourescence |
| Dergi ISSN | 1053-0509 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | Türkçe |
| Basım Tarihi | 02-2023 |
| Cilt No | 33 |
| Sayfalar | 1227 / 1236 |
| Doi Numarası | 10.1007/s10895-023-03180-9 |
| Makale Linki | http://dx.doi.org/10.1007/s10895-023-03180-9 |
| Atıf Sayıları | |
| WoS | 2 |
| SCOPUS | 2 |
