| Yazarlar |
Doç. Dr. Elçin GÜNAYDIN
Türkiye |
Gülşen Goncagül
Bursa Uludağ Üniversitesi, Türkiye |
|
Pınar Mursaloğlu Kaynar
Türkiye |
| Özet |
| Objective: Repeat breeder, which is one of the reproductive system disorders seen in cattle, is defined as the inability to conceive with three or more matings with true heat, is seen to be a breeding problem that causes serious economic losses all over the world with a decrease in reproductive efficiency and therefore a decrease in milk production. The aim of this study is to identify the aerobic microflora and antibiotic resistance profiles of dominant bacterial groups in the genital tract of repeat breeder cows that do not exhibit clinical signs of any genital system infections using conventional cultural methods. Methods: The study material comprised 32 repeat breeder cows of Holstein breed aged between four and nine years (with three or more inseminations). Specimens were collected by sterile swab. The collected swab samples were taken into tubes with Fluid Thioglycollate Medium and delivered to the laboratory under cold chain conditions. Specimens for bacteriological examination were incubated at 37 °C for 24 h. After the end of incubation, isolation and identification of colonies growing on EMB agar and blood agar medium were performed. Results: Bacteriological isolation and identification of genital canal samples revealed the isolation of 14 bacterial species mainly from the phylum Protobacteria, along with Firmicutes and Actinobacteria filaments. Among the isolated bacterial species, Escherichia coli (21.4%), Stenotrophomonas maltophilia (14.3%), Staphylococcus haemolyticus (11.9%), Staphylococcus sciuri (7.1%), Corynebacterium psuedodiphteriticum (7.1%), and Corynebacterium bovis (4.8%) were identified. In repeat breeder cows. Some of the isolated bacteria were associateted with subclinical endometritis leading to infertility in repeat breeder cows. Susceptibility of eight antibiotics against dominant bacterial groups and infertility-related bacteria were examined by antibiogram. Conclusion: It was determined that bacterial isolations such as C. bovis and C. psuedodiphteriticum from the Actinobacteria phylum and S. sciuri and S. haemolyticus from the Firmicutes phylum in samples taken from cows identified as repeat breeders are as significant as E. coli from the Protobacteria phylum. Ceftiofur demonstrated 100% effectiveness against the predominant pathogen E. coli in the vaginal microbiome. |
| Anahtar Kelimeler |
| antibiotic resistance | bacteria | cow | Repeat breeder |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | Türk Hijyen ve Deneysel Biyoloji Dergisi |
| Dergi ISSN | 0377-9777 |
| Dergi Tarandığı Indeksler | Scopus |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2023 |
| Cilt No | 80 |
| Sayı | 4 |
| Sayfalar | 513 / 522 |
| Doi Numarası | 10.5505/TURKHIJYEN.2023.44270 |
| Makale Linki | ljlvorjgerıdu |
| Atıf Sayıları |
