| Yazarlar |
Tülin Güven Gökmen
Türkiye |
|
Gülfer Yakıcı
Türkiye |
|
Yıldız Kalaycı
Türkiye |
|
Nevin Türüt
|
|
Melda Meral Öcal
Türkiye |
|
Mehmet Halıgür
Türkiye |
Doç. Dr. Elçin GÜNAYDIN
Kastamonu Üniversitesi, Türkiye |
|
Fatih Köksal
Türkiye |
| Özet |
| Background: Mycobacterium bovis is a zoonotic member of the Mycobacterium tuberculosis complex with a wide range of hosts, mainly cattle. Molecular epidemiological studies should be conducted to determine the transmission route, zoonotic risk factors, and phylogenetic relationships of M. bovis strains. Aims: This study aimed to characterize bovine and human M. bovis isolates by molecular methods. Methods: Molecular characterization and clonal relationship of strains isolated from tissue and organ samples of 76 cattle with positive tuberculin tests were collected from a slaughterhouse, and four M. bovis strains isolated from clinical materials of patients with suspected pulmonary TB isolates were analyzed using 24-locus MIRU-VNTR and spoligotyping methods. QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen) was used to determine the prevalence of latent TB infection among 21 slaughterhouse personnel including 7 veterinarians, 12 butchers, 1 caretaker, and 1 veterinary technician. Results: SB0288/SIT685 type was detected in both cattle and humans by the spoligotyping method. When evaluating MIRU-VNTR, the presence of a 100% compatible pattern between human and bovine isolates was not detected, but some human samples were found to be 91.6% similar to a bovine sample. In addition, 21 slaughterhouse workers were screened with the interferon gamma-released assay (IGRA) and a 23.8% positivity was detected. Conclusion: Clonal similarity was determined between the bovine and human isolates using the MIRU-VNTR and spoligotyping methods and IGRA positivity in the occupational group suggested that M. bovis might be associated with pulmonary tuberculosis in humans. |
| Anahtar Kelimeler |
| IGRA | Molecular epidemiology | Mycobacterium bovis |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Iranian Journal of Veterinary Research |
| Dergi ISSN | 1728-1997 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2022 |
| Cilt No | 23 |
| Sayı | 3 |
| Sayfalar | 210 / 218 |
| Doi Numarası | 10.22099/IJVR.2022.6814 |
| Makale Linki | 10.22099/IJVR.2022.42580.6186 |
| Atıf Sayıları | |
| SCOPUS | 4 |
