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Salmonella Profile in Chickens Determined by Real-time Polymerase Chain Reaction and Bacteriology from Years 2000 to 2003 in Turkey     
Yazarlar
Ayşegül Eyigör
Gülşen Goncagül
Uludağ Üniversitesi, Türkiye
Doç. Dr. Elçin GÜNAYDIN Doç. Dr. Elçin GÜNAYDIN
Türkiye
Tayfun Çarlı
Özet
From years 2000 to 2003, Salmonella was investigated from a total of 1785 samples comprised of chicken intestinal samples, cloacal swabs, drag swabs, litter samples and chick dust samples collected from 191 poultry breeding flocks belonging to 15 different chicken breeding stock companies in the Marmara region, Turkey by a SYBR green-based real-time polymerase chain reaction (SGBRT-PCR), by a probe-specific real-time polymerase chain reaction (PSRT-PCR) and by standardized bacteriology as described in the manual of National Poultry Improvement Plan and Auxiliary Provisions, United States Department of Agriculture. Between January 2000 and July 2001, Salmonella was detected at the rates of 5.87% and 4.10% out of a total of 1242 samples by SGBRT-PCR and bacteriology, respectively. From July 2001 until December 2003, Salmonella was found at rates of 11.42% and 5.52% from a total of 543 samples by PSRT-PCR and bacteriology, respectively. The dominant Salmonella serovar was determined as Salmonella enterica subsp. enterica Serovar Enteritidis (S. Enteritidis), while serogroup C1 and C2 in 2001 and serogroup E1 in 2002 were isolated as additional serovars. As a conclusion, S. Enteritidis seems to be the major problem in poultry breeding flocks in Turkey, and both of the real-time polymerase chain reaction methods were found more sensitive than standard bacteriology for the detection of Salmonella from poultry samples. © 2005 Houghton Trust Ltd.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Avian Pathology
Dergi ISSN 0307-9457
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce
Basım Tarihi 04-2005
Cilt No 34
Sayı 2
Sayfalar 101 / 105
Doi Numarası 10.1080/03079450500059461