A Capillary Polymerase Chain Reaction for Salmonella Detection from Poultry meat (Tez Yayını)
Yazarlar (3)
Doç. Dr. Elçin GÜNAYDIN Bursa Uludağ Üniversitesi, Türkiye
A. Eyigor Bursa Uludağ Üniversitesi, Türkiye
K. T. Carli Bursa Uludağ Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Letters in Applied Microbiology | ||
| Dergi ISSN | 0266-8254 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2007 |
| Cilt / Sayı / Sayfa | 44 / 1 / 24–29 | DOI | 10.1111/j.1472-765X.2006.02033.x |
| Özet |
| Aims In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. Methods and Results Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite cystine broth (SCB) artificially contaminated with 10-fold dilutions of 6 × 108 CFU ml-1 of pure Salmonella enterica ssp. enterica serovar Enteritidis 64K stock culture. Detection limits of cPCR from TTB, RVB and SCB were found as 6, 6 × 101 and 6 × 104 CFU ml-1, respectively. In addition, detection limits of bacteriology were also determined as 6 CFU ml-1 with TTB and SCB, and 6 × 101 CFU ml -1 with RVB. A total of 200 samples, consisting of 100 chicken and 100 turkey meat samples, were tested with optimized cPCR and bacteriology. Eight and six per cent of the chicken meat samples were … |
| Anahtar Kelimeler |
| Capillary polymerase chain reaction | Poultry meat | Salmonella |
| Atıf Sayıları | |
| Scopus | 11 |
| Google Scholar | 18 |