Phylo-typing of Clinical Escherichia coli Isolates Originating from Bovine Mastitis and Canine Pyometra and Urinary Tract Infection by means of Quadruplex PCR
 
Yazarlar (8)
Hamit Kaan Müştak Ankara Üniversitesi, Türkiye
Doç. Dr. Elçin GÜNAYDIN Central Veterinary Control And Research Institute, Türkiye
İnci Başak Kaya Ankara Üniversitesi, Türkiye
Merve Özdal Salar Ankara Üniversitesi, Türkiye
Orkun Babacan Ankara Üniversitesi, Türkiye
Kaan Önat Republic Of Turkey Ministry Of Food, Türkiye
Zafer Ata Military Veterinary School And Educational Central Commandership, Türkiye
Kadir Serdar Diker Ankara Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Veterinary Quarterly
Dergi ISSN 0165-2176 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce Basım Tarihi 07-2015
Cilt / Sayı / Sayfa 35 / 4 / 194–199 DOI 10.1080/01652176.2015.1068963
Özet
Background: Escherichia coli is one of the major causative agents of bovine mastitis worldwide, and is typically associated with acute, clinical mastitis. Besides this, E. coli strains which belong to the extra-intestinal pathogenic group are also the major cause of urinary tract infections and pyometra in dogs.Objectives: In this study, it was aimed to investigate phylo-groups/subgroups in 155 E. coli isolates obtained from acute bovine mastitis, 43 from urinary tract infections of dogs and 20 from canine pyometra by a formerly described triplex PCR and recently described new quadruplex polymerase chain reaction (PCR) method.Results: Group A1 (n = 118; 76%) and B1 (n = 71; 46%) were found to be the most prevalent groups by triplex and quadruplex PCR assays in mastitis isolates, respectively. Phylo-typing of 43 urinary tract isolates also revealed that most of the isolates belonged to A1 (n = 23; 54%) by triplex and …
Anahtar Kelimeler
bovine mastitis | canine pyometra | canine urinary tract infection | Escherichia coli | phylo-group | quadruplex PCR