| Yazarlar |
Nurgün Küçükboyacı
Gazi Üniversitesi, Türkiye |
|
Ayşegül Güvenç
|
|
Erdal Dinç
Ankara Üniversitesi, Türkiye |
|
Nezaket Adıgüzel
Gazi Üniversitesi, Türkiye |
Prof. Dr. Barış BANİ
Kastamonu Üniversitesi, Türkiye |
| Özet |
| New HPLC-chemometric approaches were proposed for the simultaneous chromatographic quantification of daidzein, genistein, formononetin, and biochanin A in the samples consisting of the aerial parts of Trifolium lucanicum Gasp. (Leguminosae). Partial least squares and principal component regression algorithms were applied to the multiple chromatographic data set obtained by measuring at 240, 248, 256, and 264 nm to construct HPLC-partial least squares and HPLC-principal component regression calibrations. Chromatographic separation was carried out by using a mobile phase containing methanol, acetate buffer (pH=4.75) and acetonitrile (21:58:21, v/v/v) on the reversed phase column, Supelcosil™ LC-18 (15 cmx4.6mm id). In addition, conventional HPLC based on the detection at a single wavelength was used for the determination of each compound in the extracts of T. lucanicum. The validity and applicability of the proposed HPLC-chemometric and conventional HPLC methods were performed by analyzing various synthetic plant samples. A good agreement was observed in the application of the proposed HPLC-chemometric tools to the synthetic and extracted samples of T. lucanicum. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. |
| Anahtar Kelimeler |
| HPLC-chemometric methods | Isoflavones | Leguminosae | Quantitative analysis | Trifolium lucanicum |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF SEPARATION SCIENCE |
| Dergi ISSN | 1615-9306 |
| Dergi Tarandığı Indeksler | SSCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2010 |
| Cilt No | 33 |
| Sayı | 17 |
| Sayfalar | 2558 / 2567 |
| Doi Numarası | 10.1002/jssc.201000273 |
| Atıf Sayıları | |
| WoS | 5 |
| SCOPUS | 7 |
