| Yazarlar |
Nurgün Küçükboyacı
Gazi Üniversitesi, Türkiye |
|
Onur Kadıoğlu
|
|
Nezaket Adıgüzel
Gazi Üniversitesi, Türkiye |
|
Uğur Tamer
Gazi Üniversitesi, Türkiye |
|
Ayşegül Güvenç
|
Prof. Dr. Barış BANİ
Kastamonu Üniversitesi, Türkiye |
| Özet |
| In this study, the methanol and acid hydrolyzed methanol extracts prepared from the aerial parts of two varieties of Trifolium pratense (red clover) collected from different parts of Turkey, namely T. pratense L. var. pratense and T. pratense L. var. sativum Schreb., have been analyzed to determine the concentrations of four isoflavones, daidzein (D), genistein (G), formononetin (F) and biochanin A (B). A RP-HPLC-UV method for the determination of these isoflavones in red clover was developed. Chromatographic separation was carried out by using a gradient system consisting of 0.3 % acetic acid and methanol on a reversed phase column, Supelcosil™ LC-18 (15 cm x 4.6 mm id, 5μm). The highest content of total D, G, F, and B were found as 0.0447, 0.0115, 0.0251 and 0.0039 % in T. pratense var. pratense, respectively. Antioxidant capacity of the methanol extracts of aerial parts of plant samples was also studied. Studied extracts were found to possess some antioxidant activities, as determined by the DPPH free radical scavenging test, thiobarbituric acid and trolox-equivalent antioxidant capacity assays. The phenolic contents of the samples were determined using Folin-Ciocalteu's reagent. |
| Anahtar Kelimeler |
| Antioxidant activity | Fabaceae | HPLC | Isoflavones | Trifolium pratense |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | Turkish Journal of Pharmaceutical |
| Dergi ISSN | 1304-530X |
| Dergi Tarandığı Indeksler | Chemical Abstracts, EMBASE, Analytical Abstracts, ULAKBİM, TR Dizin, International Pharmaceutical Abstracts, MAPA, Türkiye Atıf Dizini, SCOPUS SJR |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2013 |
| Cilt No | 10 |
| Sayı | 3 |
| Sayfalar | 463 / 472 |
