Determination of Triploidy in Rainbow Trout, Oncorhynchus mykiss using Erythrocyte Measurements
Yazarlar (6)
Arş. Gör. Dr. Osman Nezih KENANOĞLU Kastamonu Üniversitesi, Türkiye
Prof. Dr. Sevdan Yılmaz Çanakkale Onsekiz Mart Üniversitesi, Türkiye
Nergiz Soytaş
Prof. Dr. Sebahattin Ergün Çanakkale Onsekiz Mart Üniversitesi, Türkiye
Prof. Dr. Cüneyt Akı Çanakkale Onsekiz Mart Üniversitesi, Türkiye
Fatih Tapan
| Makale Türü |
Özgün Makale
(Diğer hakemli uluslarası dergilerde yayınlanan tam makale)
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| Dergi Adı | Marine Science and Technology Bulletin | ||
| Dergi ISSN | 2147-9666 | ||
| Dergi Tarandığı Indeksler | cabi agris crossref road drji esji asos | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2012 |
| Cilt / Sayı / Sayfa | 1 / 1 / 17–19 | DOI | – |
| Makale Linki | https://dergipark.org.tr/tr/pub/masteb/issue/22359/239461 | ||
| UAK Araştırma Alanları |
Su Ürünleri
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| Özet |
| In this study, effects of thermal shock (26°C) on Oncorhynchus mykiss egg at different duration time (5, 10, 15 and 20 min.) to triploid rate was determined. Level of triploid induction for each treatment was evaluated by erythrocyte measurements. For this purpose, the caudal peduncles of 40fry for each group were cut off and blood smears prepared. The cell and nucleus lengths (major and minor axis) of 30 erythrocytes for each individual were determined with the aid of a microscope camera (with ScopImage 9.0®) and using a micrometer. There was considerable variation in major axisof erythrocytes cell and nucleus both within individual fish and among individuals of a given ploidy. In the major axis length of erythrocyte, cell and nucleus of 10, 15, 20 min thermal shocked groups were statistically significant compared to the control groups (p<0.05). The presented study demonstrated that the increases of triploid yields are correlated to length oferythrocyte major axis. In conclusion, the best triploid yield for O. mykiss was at 26°C for 15 min initiated 15 min after fertilization. |
| Anahtar Kelimeler |
