| Yazarlar |
Zuhal Önder
Erciyes Üniversitesi, Türkiye |
|
Emrah Şimşek
Erciyes Üniversitesi, Türkiye |
|
Önder Düzlü
Erciyes Üniversitesi, Türkiye |
|
Gamze Yetişmiş
Erciyes Üniversitesi, Türkiye |
|
Arif Çiloğlu
Erciyes Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Mübeccel ATELGE
Kastamonu Üniversitesi, Türkiye |
|
Nesrin Delibaşı Kökçü
|
|
Abdullah İnci
Erciyes Üniversitesi, Türkiye |
|
Alparslan Yıldırım
Erciyes Üniversitesi, Türkiye |
| Özet |
| The molecular prevalence and genotypes of Giardia duodenalis in cattle were investigated. A total of 450 fecal samples were collected from cattle in three provinces of Central Anatolia from August 2017 to July 2019. Genomic DNA was extracted from the fecal samples and used in molecular analysis carried out by nested PCR analyses of the β-giardin (bg) gene of G. duodenalis. Positive samples were further analyzed by nested PCR at two gene loci (triosephosphate isomerase (tpi) and glutamate dehydrogenase (gdh)) for genotyping of G. duodenalis isolates. PCR analyses of the bg gene indicated that the overall prevalence of G. duodenalis was 30.2%. However, lower rates were determined with PCR analyses for gdh and tpi loci. The sequence analyses of the bg, gdh, and tpi genes revealed the presence of zoonotic assemblage A and livestock-specific assemblage E. Combined-sequence analyses revealed that assemblage E was the most common in the study area. Our study provides the first data on the wide prevalence of livestock-specific assemblages E in cattle in Turkey. The prevalence of assemblage A in cattle also reveals the importance of cattle for zoonotic transmission of giardiasis in Turkey. |
| Anahtar Kelimeler |
| Assemblage | Cattle | Central Anatolia | Giardia duodenalis | Glutamate dehydrogenase | Molecular prevalence | Triosephosphate isomerase | β-Giardin |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | PARASITOLOGY RESEARCH |
| Dergi ISSN | 0932-0113 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | Türkçe |
| Basım Tarihi | 09-2020 |
| Cilt No | 119 |
| Sayı | 9 |
| Sayfalar | 2927 / 2934 |
| Doi Numarası | 10.1007/s00436-020-06771-8 |
| Makale Linki | http://dx.doi.org/10.1007/s00436-020-06771-8 |
