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First Molecular Detection and Phylogenetic Analyses of Zoonotic Giardia intestinalis in Horses in Turkey      
Yazarlar
Kübra Demircan
Zuhal Önder
Erciyes Üniversitesi, Türkiye
Önder Düzlü
Erciyes Üniversitesi, Türkiye
Alparslan Yıldırım
Erciyes Üniversitesi, Türkiye
Dr. Öğr. Üyesi Mübeccel ATELGE Dr. Öğr. Üyesi Mübeccel ATELGE
Kastamonu Üniversitesi, Türkiye
Arif Çiloğlu
Erciyes Üniversitesi, Türkiye
Gamze Yetişmiş
Erciyes Üniversitesi, Türkiye
Abdullah İnci
Erciyes Üniversitesi, Türkiye
Özet
The goal of our study was to investigate the molecular prevalence of Giardia intestinalis in naturally infected horses in Kayseri, Central Anatolia Region in Turkey, to determine the molecular characterization of the obtained isolates and to exhibit the potential role of horses in zoonotic transmission of G. intestinalis. Fecal samples were randomly collected from totally 150 horses with clinically healthy between March and June of 2018. After the genomic DNA extractions, 25 (16.6%) of the 150 fecal samples, were found positive for G. intestinalis by nested PCR analyses of β-giardin gene. Phylogenetic analysis of the β-giardin gene sequences of G. intestinalis showed that the sequences detected in this study belonged to assemblage A that is regarded as zoonotic. Our study is the first report on the presence of G. intestinalis in horses in Turkey. The findings of the present study indicate that future research studies are required to determine molecular epidemiology and geographical distribution of G. intestinalis infections in horses nationwide. In addition, this study also may be helpful to assess the zoonotic potential for public health of G. intestinalis infections.
Anahtar Kelimeler
Giardia intestinalis | Horse | Molecular characterization | Turkey | Zoonosis
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı JOURNAL OF EQUINE VETERINARY SCIENCE
Dergi ISSN 0737-0806
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q3
Makale Dili Türkçe
Basım Tarihi 09-2019
Cilt No 80
Sayı 1
Sayfalar 56 / 60
Doi Numarası 10.1016/j.jevs.2019.06.017
Makale Linki http://dx.doi.org/10.1016/j.jevs.2019.06.017