Molecular detection and identification of Wolbachia endosymbiont in fleas (Insecta: Siphonaptera)
Yazarlar (7)
Zuhal Önder Erciyes Üniversitesi, Türkiye
Arif Çiloğlu Erciyes Üniversitesi, Türkiye
Önder Düzlü Erciyes Üniversitesi, Türkiye
Alparslan Yıldırım Erciyes Üniversitesi, Türkiye
Dr. Öğr. Üyesi Mübeccel ATELGE Kastamonu Üniversitesi, Türkiye
Gamze Yetişmiş Erciyes Üniversitesi, Türkiye
Abdullah İnci Erciyes Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Folia Microbiologica (Q3) | ||
| Dergi ISSN | 0015-5632 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2019 |
| Cilt / Sayı / Sayfa | 64 / 6 / 789–796 | DOI | 10.1007/s12223-019-00692-5 |
| Makale Linki | http://dx.doi.org/10.1007/s12223-019-00692-5 | ||
| Özet |
| The aim of this study was to determine the presence and prevalence of Wolbachia bacteria in natural population of fleas (Insecta: Siphonaptera) in Turkey, and to exhibit the molecular characterization and the phylogenetic reconstruction at the positive isolates with other species in GenBank, based on 16S rDNA sequences. One hundred twenty-four flea samples belonging to the species Ctenocephalides canis, C. felis, and Pulex irritans were collected from animal shelters in Kayseri between January and August 2017. All flea species were individually screened for the presence of Wolbachia spp. by polymerase chain reaction (PCR) targeting the 16S ribosomal RNA gene. According to PCR analyses, Wolbachia spp. were found prevalent in C. canis and P. irritans fleas, while it was not detected in the C. felis species. Totally, 20 isolates were purified from agarose gel and sequenced with the same primers for … |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 11 |
| Scopus | 12 |
| Google Scholar | 16 |